Burkholderia pseudomallei K96243, BPSL2281 (lysS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL2281
Name
lysS
Replicon chromosome 1
Genomic location 2749035 - 2750561 (+ strand)

Cross-References

RefSeq YP_108877.1
GI 53719891
Entrez 3092883
GI 53719891
NCBI Locus Tag BPSL2281
RefSeq YP_108877.1,GeneID
UniProtKB Acc Q63SN9
UniProtKB ID SYK_BURPS

Product

Feature Type CDS
Coding Frame 1
Product Name
lysyl-tRNA synthetase
Synonyms
Evidence for Translation
Charge (pH 7) -11.86
Kyte-Doolittle Hydrophobicity Value -0.302
Molecular Weight (kDa) 57143.4
Isoelectric Point (pI) 5.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
quantitative mass spectrometry evidence
Extracellular Class 3
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4EX5 TRANSFERASE 04/29/12 Crystal structure of lysyl-tRNA synthetase LysRS from Burkholderia thailandensis bound to lysine BURKHOLDERIA THAILANDENSIS 2.4 X-RAY DIFFRACTION 96.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG002011 (653 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL2281
Search term: lysS

Human Homologs

Ensembl 97, assembly GRCh38.p12
lysyl-tRNA synthetase [Source:HGNC Symbol;Acc:HGNC:6215]
E-value: 6.7e-93
Percent Identity: 42.3

Human Homologs

Ensembl 97, assembly GRCh38.p12
lysyl-tRNA synthetase [Source:HGNC Symbol;Acc:HGNC:6215]
E-value: 6.7e-93
Percent Identity: 42.3

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268