Burkholderia xenovorans LB400, Bxe_A1420

Annotation Updates

Date	Description	Acknowledgements
Jan 25, 2011	New product name confidence: Class 2. New comment : Expressed as a hexa-histidine-tagged fusion in Escherichia coli BL21 Star (DE3). ALDHC in complex with NADPH bound in the cofactor binding pocket and an ordered fragment of a polyethylene glycol molecule bound in the substrate tunnel, to 1.6 A resolution. Belongs to space group P1. The ALDHC monomer comprises three distinct domains, an N-terminal cofactor (NAD/P+)-binding domain, a catalytic domain, and an oligomerization domain. New EC Number: 1.2.1.5. New GO Accession: GO:0004030(IDA). New PubMed reference: 18462753.	Literature review of BRENDA website by Geoffrey L. Winsor (Brinkman Lab). Simon Fraser University, Canada
Jan 25, 2011	New comment : ALDHC is preferentially active towards linear medium-chain to long-chain aldehydes as compared to branched-chain, short-chain or aromatic aldehydes. Reorientation of an extended loop (Asn478-Pro490) is responsible for the constricted structure of the substrate tunnel, with the side chain of Asn478 imposing steric restrictions on branched-chain and aromatic aldehydes. A key glycine (Gly104) positioned at the mouth of the tunnel allows for maximum tunnel depth required to bind the linear medium to long aliphatic chain of the native substrate.	Literature review of BRENDA website by Geoffrey L. Winsor (Brinkman Lab). Simon Fraser University, Canada
Jan 25, 2011	New product name confidence: Class 2. New EC Number: 1.2.1.5. New GO accession: GO:0004030 [IDA]. New evidence reference (PMID): 18462753.	Literature review of BRENDA website by Geoffrey L. Winsor (Brinkman Lab). Simon Fraser University, Canada