Burkholderia cenocepacia J2315, BCAM0855 (bceC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAM0855
NCBI Locus TagQU43_RS58660
Name
bceC
Replicon chromosome 2
Genomic location 943948 - 945360 (+ strand)
CommentMember of the bce-I gene cluster

Cross-References

RefSeq WP_006497289.1
GI 206562712
Entrez 6930371
GI 206562712
INSDC CAR54714.1
NCBI Locus Tag QU43_RS58660
NCBI Old Locus Tag BCAM0855
RefSeq WP_006497289.1,GeneID
UniParc UPI00017B99EF
UniProtKB Acc B4EMQ8
UniProtKB ID B4EMQ8_BURCJ
UniRef100 UniRef100_B4EMQ8
UniRef50 UniRef50_O86422
UniRef90 UniRef90_B4EMQ8

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-Glucose Dehydrogenase BceC
Product Name Confidence: Class 2
Synonyms UDP-glucose dehydrogenase
Evidence for Translation
Charge (pH 7) -9.20
Kyte-Doolittle Hydrophobicity Value -0.162
Molecular Weight (kDa) 51228.5
Isoelectric Point (pI) 5.27

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2Y0D OXIDOREDUCTASE 12/02/10 BceC mutation Y10K BURKHOLDERIA CEPACIA 2.8 X-RAY DIFFRACTION 97.7
2Y0C OXIDOREDUCTASE 12/02/10 BceC mutation Y10S BURKHOLDERIA CEPACIA 1.75 X-RAY DIFFRACTION 97.7
2Y0E OXIDOREDUCTASE 12/02/10 BceC and the final step of UGDs reaction BURKHOLDERIA CEPACIA 1.75 X-RAY DIFFRACTION 97.9

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 587 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000893 (1284 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAM0855
Search term: bceC

Human Homologs

Ensembl 97, assembly GRCh38.p12
UDP-glucose 6-dehydrogenase [Source:HGNC Symbol;Acc:HGNC:12525]
E-value: 9.3e-65
Percent Identity: 35.9
Ensembl 97, assembly GRCh38.p12
UDP-glucose 6-dehydrogenase [Source:HGNC Symbol;Acc:HGNC:12525]
E-value: 9.3e-65
Percent Identity: 35.9

References

Identification and physical organization of the gene cluster involved in the biosynthesis of Burkholderia cepacia complex exopolysaccharide.
Moreira LM, Videira PA, Sousa SA, Leitão JH, Cunha MV, Sá-Correia I
Biochem Biophys Res Commun 2003 Dec 12;312(2):323-33
PubMed ID: 14637140
Contributions of two UDP-glucose dehydrogenases to viability and polymyxin B resistance of Burkholderia cenocepacia.
Loutet SA, Bartholdson SJ, Govan JRW, Campopiano DJ, Valvano MA
Microbiology 2009 Jun;155(Pt 6):2029-2039
PubMed ID: 19383677
Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions.
Ferreira AS, Leitão JH, Silva IN, Pinheiro PF, Sousa SA, Ramos CG, Moreira LM
Appl Environ Microbiol 2010 Jan;76(2):441-50
PubMed ID: 19948863
Structure of Burkholderia cepacia UDP-glucose dehydrogenase (UGD) BceC and role of Tyr10 in final hydrolysis of UGD thioester intermediate.
Rocha J, Popescu AO, Borges P, Mil-Homens D, Moreira LM, Sá-Correia I, Fialho AM, Frazão C
J Bacteriol 2011 Aug;193(15):3978-87
PubMed ID: 21602353
Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose dehydrogenase from Burkholderia cepacia IST408.
Rocha J, Popescu AO, Sá-Correia I, Fialho AM, Frazão C
Acta Crystallogr Sect F Struct Biol Cryst Commun 2010 Mar 1;66(Pt 3):269-71
PubMed ID: 20208157