Burkholderia cenocepacia J2315, BCAL3121 (wbiG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAL3121
NCBI Locus TagQU43_RS52335
Name
wbiG
Replicon chromosome 1
Genomic location 3412044 - 3413021 (- strand)
CommentBelongs to the O antigen biosynthesis gene cluster

Cross-References

RefSeq YP_002232232.1
GI 206561467
Entrez 6934241
GI 206561467
INSDC CAR53445.1
NCBI Locus Tag QU43_RS52335
NCBI Old Locus Tag BCAL3121
RefSeq YP_002232232.1
UniParc UPI00017B998B
UniProtKB Acc B4ECE2
UniProtKB ID B4ECE2_BURCJ
UniRef100 UniRef100_B4ECE2
UniRef50 UniRef50_A9AF88
UniRef90 UniRef90_B4ECE2

Product

Feature Type CDS
Coding Frame 1
Product Name
putative nucleotide sugar epimerase/dehydratase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 2.33
Kyte-Doolittle Hydrophobicity Value -0.016
Molecular Weight (kDa) 34977.6
Isoelectric Point (pI) 7.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database BGD
Category Lipopolysaccharide O antigen
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes wbiG mutant attenuated for virulence in agar bead model of chronic lung infection
Reference 15213146

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 632 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000740 (630 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAL3121
Search term: wbiG

References

Reconstitution of O-specific lipopolysaccharide expression in Burkholderia cenocepacia strain J2315, which is associated with transmissible infections in patients with cystic fibrosis.
Ortega X, Hunt TA, Loutet S, Vinion-Dubiel AD, Datta A, Choudhury B, Goldberg JB, Carlson R, Valvano MA
J Bacteriol 2005 Feb;187(4):1324-33
PubMed ID: 15687196
Insect Gut Symbiont Susceptibility to Host Antimicrobial Peptides Caused by Alteration of the Bacterial Cell Envelope.
Kim JK, Son DW, Kim CH, Cho JH, Marchetti R, Silipo A, Sturiale L, Park HY, Huh YR, Nakayama H, Fukatsu T, Molinaro A, Lee BL
J Biol Chem 2015 Aug 21;290(34):21042-53
PubMed ID: 26116716