Burkholderia cenocepacia J2315, BCAL1929 (arnT)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse

Gene Feature Overview

Strain Burkholderia cenocepacia J2315
Locus Tag
NCBI Locus TagQU43_RS46190
Replicon chromosome 1
Genomic location 2128443 - 2130119 (+ strand)
CommentIn PMID:27597847: arnT, arnB, arnC, arnA1, arnA2, and BCAL1935 located in the arn gene cluster harbored significantly low frequencies of transposon insertions (TraDIS). They were therefore categorized as essential by the authors.


RefSeq WP_006482861.1
GI 206560293
Entrez 6932119
GI 206560293
INSDC CAR52230.1
NCBI Locus Tag QU43_RS46190
NCBI Old Locus Tag BCAL1929
RefSeq WP_006482861.1,GeneID
UniParc UPI00017B921E
UniProtKB Acc B4EB15
UniRef100 UniRef100_A0A0D1BVQ3
UniRef50 UniRef50_E5AQP3
UniRef90 UniRef90_A9AG56


Feature Type CDS
Coding Frame 1
Product Name
4-amino-4-deoxy-L-arabinose transferase ArnT
Product Name Confidence: Class 1
Synonyms aminoarabinose transferase
putative undecaprenyl phosphate-alpha-4-amino-4-deoxy-l-arabinose arabinosy transferase
Evidence for Translation
Charge (pH 7) 18.09
Kyte-Doolittle Hydrophobicity Value 0.493
Molecular Weight (kDa) 62686.3
Isoelectric Point (pI) 10.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
direct assay evidence used in manual assertion
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 249 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG001691 (653 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BCAL1929
Search term: arnT


A putative gene cluster for aminoarabinose biosynthesis is essential for Burkholderia cenocepacia viability.
Ortega XP, Cardona ST, Brown AR, Loutet SA, Flannagan RS, Campopiano DJ, Govan JR, Valvano MA
J Bacteriol 2007 May;189(9):3639-44
PubMed ID: 17337576
PubMed ID: 22742453
ArnT proteins that catalyze the glycosylation of lipopolysaccharide share common features with bacterial N-oligosaccharyltransferases.
Tavares-Carreón F, Fathy Mohamed Y, Andrade A, Valvano MA
Glycobiology 2016 Mar;26(3):286-300
PubMed ID: 26515403
Burkholderia cenocepacia and Salmonella enterica ArnT proteins that transfer 4-amino-4-deoxy-l-arabinose to lipopolysaccharide share membrane topology and functional amino acids.
Tavares-Carreón F, Patel KB, Valvano MA
Sci Rep 2015 Jun 1;5:10773
PubMed ID: 26030265
An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor.
Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR
J Biol Chem 2001 Nov 16;276(46):43122-31
PubMed ID: 11535604
Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS.
Wong YC, Abd El Ghany M, Naeem R, Lee KW, Tan YC, Pain A, Nathan S
Front Microbiol 2016;7:1288
PubMed ID: 27597847