Burkholderia thailandensis E264 ATCC 700388, BTH_I2957 (tssA-1)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse

Gene Feature Overview

Strain Burkholderia thailandensis E264 ATCC 700388
Locus Tag
NCBI Locus TagBTH_RS27275
Replicon chromosome I
Genomic location 3402329 - 3403438 (- strand)
Transposon Mutants 4 transposon mutants in E264 ATCC 700388
CommentThis gene is part of cluster known as the type VI secretion system gene cluster tss-1 based on naming convention used for B. pseudomallei described in PMID:17660433, however unlike B. pseudomallei, the numbers following the vgrG and hcp gene match the cluster number (PMID:20865170).


RefSeq YP_443464.1
GI 83718946
Entrez 3848350
GI 83718946
INSDC ABC36834.1
NCBI Locus Tag BTH_RS27275
NCBI Old Locus Tag BTH_I2957
RefSeq YP_443464.1
UniParc UPI0000667D61
UniProtKB Acc Q2SUD5
UniRef100 UniRef100_Q2SUD5
UniRef50 UniRef50_A1UZK2
UniRef90 UniRef90_A1UZK2


Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssA-1
Product Name Confidence: Class 2
Synonyms TssA-1
ImpA-like N-terminal family protein
Evidence for Translation
Charge (pH 7) -18.63
Kyte-Doolittle Hydrophobicity Value -0.431
Molecular Weight (kDa) 40832.1
Isoelectric Point (pI) 4.64

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6H8E TRANSPORT PROTEIN 08/02/18 Truncated derivative of the C-terminal domain of the TssA component of the type VI secretion system from Burkholderia cenocepacia BURKHOLDERIA CENOCEPACIA H111 2.35 X-RAY DIFFRACTION 90.9

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 75 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000373 (645 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BTH_I2957
Search term: tssA-1


Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD
PLoS Pathog 2010 Aug 26;6(8):e1001068
PubMed ID: 20865170
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434