Burkholderia pseudomallei K96243, BPSS1830 (bceF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS1830
Name
bceF
Replicon chromosome 2
Genomic location 2488808 - 2491027 (- strand)
CommentMember of the bce-I gene cluster

Cross-References

RefSeq YP_111834.1
GI 53722849
Entrez 3095477
GI 53722849
INSDC CAH39306.1
NCBI Locus Tag BPSS1830
RefSeq YP_111834.1,GeneID
UniParc UPI000043BE43
UniProtKB Acc Q63J88
UniProtKB ID Q63J88_BURPS
UniRef100 UniRef100_A0A069BAV0
UniRef50 UniRef50_A9ANS7
UniRef90 UniRef90_Q2T7V2

Product

Feature Type CDS
Coding Frame 1
Product Name
tyrosine kinase BceF
Product Name Confidence: Class 2
Synonyms exopolysaccharide biosynthesis-like tyrosine-protein kinase
Evidence for Translation
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (39.3 kDa, pI 4.74, PMID:21960737)
Charge (pH 7) 0.38
Kyte-Doolittle Hydrophobicity Value -0.057
Molecular Weight (kDa) 79245.0
Isoelectric Point (pI) 7.09

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 476 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG004851 (638 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1830
Search term: bceF

Human Homologs

References

Functional analysis of Burkholderia cepacia genes bceD and bceF, encoding a phosphotyrosine phosphatase and a tyrosine autokinase, respectively: role in exopolysaccharide biosynthesis and biofilm formation.
Ferreira AS, Leitão JH, Sousa SA, Cosme AM, Sá-Correia I, Moreira LM
Appl Environ Microbiol 2007 Jan;73(2):524-34
PubMed ID: 17114319
Comparative transcriptomic analysis of the Burkholderia cepacia tyrosine kinase bceF mutant reveals a role in tolerance to stress, biofilm formation, and virulence.
Ferreira AS, Silva IN, Oliveira VH, Becker JD, Givskov M, Ryan RP, Fernandes F, Moreira LM
Appl Environ Microbiol 2013 May;79(9):3009-20
PubMed ID: 23435894
The tyrosine kinase BceF and the phosphotyrosine phosphatase BceD of Burkholderia contaminans are required for efficient invasion and epithelial disruption of a cystic fibrosis lung epithelial cell line.
Ferreira AS, Silva IN, Fernandes F, Pilkington R, Callaghan M, McClean S, Moreira LM
Infect Immun 2015 Feb;83(2):812-21
PubMed ID: 25486990
Tyrosine Phosphorylation and Dephosphorylation in Burkholderia cenocepacia Affect Biofilm Formation, Growth under Nutritional Deprivation, and Pathogenicity.
Andrade A, Tavares-Carreón F, Khodai-Kalaki M, Valvano MA
Appl Environ Microbiol 2016 Feb 1;82(3):843-56
PubMed ID: 26590274
Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions.
Ferreira AS, Leitão JH, Silva IN, Pinheiro PF, Sousa SA, Ramos CG, Moreira LM
Appl Environ Microbiol 2010 Jan;76(2):441-50
PubMed ID: 19948863