Burkholderia pseudomallei K96243, BPSS1548 (bsaL)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
Locus Tag
Synonym: sctF
Replicon chromosome 2
Genomic location 2103000 - 2103269 (+ strand)
CommentThis gene is part of the type III secretion system T3SS-3 (bsa) gene locus.


RefSeq YP_111554.1
GI 53722569
Entrez 3095549
GI 53722569
INSDC CAH39021.1
NCBI Locus Tag BPSS1548
RefSeq YP_111554.1,GeneID
UniParc UPI000043BD2E
UniProtKB Acc Q63K18
UniRef100 UniRef100_A0A069BA05
UniRef50 UniRef50_Q2T727
UniRef90 UniRef90_Q2T727


Feature Type CDS
Coding Frame 1
Product Name
type III secretion system outer rod/major needle component BsaL (T3SS-3)
Product Name Confidence: Class 2
Synonyms Type III secretion system protein
Evidence for Translation
Charge (pH 7) -2.02
Kyte-Doolittle Hydrophobicity Value -0.592
Molecular Weight (kDa) 9893.9
Isoelectric Point (pI) 4.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2G0U UNKNOWN FUNCTION 02/13/06 Solution Structure of Monomeric BsaL, the Type III Secretion Needle Protein of Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI NOT SOLUTION NMR 100.0

Virulence Evidence

Source 1
Database VFDB : VF0428
Category T3SS-3
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Reference 17984080

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 12 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019858 (317 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1548
Search term: bsaL


Unraveling type III secretion systems in the highly versatile Burkholderia pseudomallei.
Sun GW, Gan YH
Trends Microbiol 2010 Dec;18(12):561-8
PubMed ID: 20951592
Type III Secretion in the Melioidosis Pathogen <i>Burkholderia pseudomallei</i>.
Vander Broek CW, Stevens JM
Front Cell Infect Microbiol 2017;7:255
PubMed ID: 28664152
Solution structure of monomeric BsaL, the type III secretion needle protein of Burkholderia pseudomallei.
Zhang L, Wang Y, Picking WL, Picking WD, De Guzman RN
J Mol Biol 2006 Jun 2;359(2):322-30
PubMed ID: 16631790
Differences in the electrostatic surfaces of the type III secretion needle proteins PrgI, BsaL, and MxiH.
Wang Y, Ouellette AN, Egan CW, Rathinavelan T, Im W, De Guzman RN
J Mol Biol 2007 Aug 31;371(5):1304-14
PubMed ID: 17617421
The response of type three secretion system needle proteins MxiHDelta5, BsaLDelta5, and PrgIDelta5 to temperature and pH.
Barrett BS, Picking WL, Picking WD, Middaugh CR
Proteins 2008 Nov 15;73(3):632-43
PubMed ID: 18491382