GEO Dataset
| GEO Dataset | Dataset details |
| GDS ID | 200012663 |
| GDS Accession | GSE12663 |
| Title | virulence determinants of Francisella tularensis revealed by transcriptional profiling inside macrophages |
| Summary | The highly infectious bacterium Francisella tularensis is a facultative intracellular pathogen, whose virulence requires proliferation inside host cells, including macrophages. Although some Francisella determinants of intracellular growth have been identified, much remains to be understood about the pathogenesis of this organism. In particular, how Francisella responds to its intracellular environment could provide clues about its intracellular biology and reveal pathogenic determinants based on their intracellular expression profiles. Here we have performed a global transcriptional profiling of the highly virulent F. tularensis subsp. tularensis Schu S4 strain during its intracellular cycle within primary murine macrophages. Phagocytosed bacteria rapidly responded to their intracellular environment and progressively altered their transcriptional profile over time. Differential gene expression profiles were revealed that correlated with specific intracellular locations of the bacteria. Upregulation of general and oxidative stress response genes was a hallmark of the early phagosomal and late endosomal stages, while induction of a subset of transport and metabolic genes characterized the cytosolic replication stages. Expression of the Francisella Pathogenicity Island (FPI) genes, the functions of which are associated with intracellular proliferation, increased during the intracellular cycle. Similarly, 27 chromosomal loci putatively encoding hypothetical, secreted, outer membrane proteins or transcriptional regulators were identified as upregulated during the intracellular cycle. In-frame deletion of FTT0383, the Schu S4 ortholog of fevR, of FTT0369c or FTT1676 abolished the ability of Schu S4 to either survive or proliferate intracellularly, demonstrating that bacterial factors of intracellular pathogenesis can be identified based on their intracellular expression profile. In conclusion, establishing the intracellular transcriptome of Francisella has revealed important aspects of its intracellular biology and identified novel virulence determinants of this pathogen. Keywords: Time series |
| Expression Profiles | 0 |
| Number of Samples | 32 |
| GEO Series | GSE12663 |
| Reference | PMID:19388904 |
| GDS Data Type | Expression profiling by array |
| Taxon | Brucella suis; Borrelia hermsii; Burkholderia mallei; Francisella tularensis subsp. tularensis SCHU S4; Francisella tularensis; Coxiella burnetii; Rickettsia prowazekii |
Platforms
| Name | Affymetrix RML Custom Pathogenic chip 2 |
| Technology Type | in situ oligonucleotide |
| Accession | GPL7246 |
GEO Samples
| GEO Accession | Condition |
|---|---|
| GSM317884 | time point zero negative control rep2 |
| GSM317877 | time point sixteen rep 1 |
| GSM317888 | time point twenty-four negative control rep 2 |
| GSM317868 | time point four rep 1 |
| GSM317872 | time point eight rep 2 |
| GSM317867 | time point two rep 3 |
| GSM317865 | time point two rep 1 |
| GSM317889 | time point twenty-four negative control rep 3 |
| GSM317879 | time point sixteen rep 3 |
| GSM317887 | time point twenty-four negative control rep 1 |
| GSM317866 | time point two rep 2 |
| GSM317886 | time point zero negative control rep4 |
| GSM317871 | time point eight rep 1 |
| GSM317862 | time point one rep 1 |
| GSM317863 | time point one rep 2 |
| GSM317861 | time point zero rep 3 |
| GSM317885 | time point zero negative control rep3 |
| GSM317870 | time point four rep 3 |
| GSM317876 | time point twelve rep 3 |
| GSM317873 | time point eight rep 3 |
| GSM317869 | time point four rep 2 |
| GSM317878 | time point sixteen rep 2 |
| GSM317874 | time point twelve rep 1 |
| GSM317859 | time point zero rep 1 |
| GSM317880 | time point twenty-four rep 1 |
| GSM317882 | time point twenty-four rep 3 |
| GSM317883 | time point zero negative control rep1 |
| GSM317875 | time point twelve rep 2 |
| GSM317881 | time point twenty-four rep 2 |
| GSM317860 | time point zero rep 2 |
| GSM317864 | time point one rep 3 |
| GSM317890 | time point twenty-four negative control rep 4 |