Burkholderia cenocepacia J2315, BCAS0208 (afcE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAS0208
NCBI Locus TagQU43_RS69720
Name
afcE
Replicon chromosome 3
Genomic location 224426 - 226276 (- strand)

Cross-References

RefSeq WP_012493697.1
GI 197295058
Entrez 6804112
GI 197295058
INSDC CAR57140.1
NCBI Locus Tag QU43_RS69720
NCBI Old Locus Tag BCAS0208
RefSeq WP_012493697.1,GeneID
UniParc UPI00017BB231
UniProtKB Acc B4EPX4
UniProtKB ID B4EPX4_BURCJ
UniRef100 UniRef100_U1XBS1
UniRef50 UniRef50_G7HPT7
UniRef90 UniRef90_G7HPT7

Product

Feature Type CDS
Coding Frame 1
Product Name
AfcE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -4.96
Kyte-Doolittle Hydrophobicity Value -0.116
Molecular Weight (kDa) 68280.1
Isoelectric Point (pI) 6.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 404 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG005261 (393 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAS0208
Search term: afcE
Search term: AfcE

Human Homologs

Ensembl 97, assembly GRCh38.p12
acyl-CoA dehydrogenase family member 8 [Source:HGNC Symbol;Acc:HGNC:87]
E-value: 7.2e-17
Percent Identity: 24.9

References

Role of Burkholderia cenocepacia afcE and afcF genes in determining lipid-metabolism-associated phenotypes.
Subramoni S, Agnoli K, Eberl L, Lewenza S, Sokol PA
Microbiology 2013 Mar;159(Pt 3):603-614
PubMed ID: 23306671
Burkholderia cenocepacia ShvR-regulated genes that influence colony morphology, biofilm formation, and virulence.
Subramoni S, Nguyen DT, Sokol PA
Infect Immun 2011 Aug;79(8):2984-97
PubMed ID: 21690240
The afc antifungal activity cluster, which is under tight regulatory control of ShvR, is essential for transition from intracellular persistence of Burkholderia cenocepacia to acute pro-inflammatory infection.
Gomes MC, Tasrini Y, Subramoni S, Agnoli K, Feliciano JR, Eberl L, Sokol P, O'Callaghan D, Vergunst AC
PLoS Pathog 2018 Dec;14(12):e1007473
PubMed ID: 30513124