Burkholderia cenocepacia J2315, BCAM0925

Gene Feature Overview

Strain	Burkholderia cenocepacia J2315 GCF_000009485.1|latest
Locus Tag	BCAM0925
NCBI Locus Tag	QU43_RS59000
Name
Replicon	chromosome 2
Genomic location	1020273 - 1021703 (- strand)
Comment	Major RND-8 substrate is tobramycin (in biofilm grown cells)
Comment	Member of rnd-8 operon encoding Resistance-Nodulation-Cell Division (RND) efflux pump RND-8

Cross-References

RefSeq	WP_006489053.1
GI	206562780
Entrez	6928660
GI	206562780
INSDC	CAR54782.1
NCBI Locus Tag	QU43_RS59000
NCBI Old Locus Tag	BCAM0925
RefSeq	WP_006489053.1,GeneID
UniParc	UPI00017B9C8C
UniProtKB Acc	B4EF70
UniProtKB ID	B4EF70_BURCJ
UniRef100	UniRef100_B4EF70
UniRef50	UniRef50_Q8XT06
UniRef90	UniRef90_A0A095ECA9

Product

Feature Type	CDS
Coding Frame	1
Product Name	RND-8 efflux system membrane fusion protein Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7)	6.71
Kyte-Doolittle Hydrophobicity Value	-0.198
Molecular Weight (kDa)	51713.0
Isoelectric Point (pI)	9.86

Subcellular localization

Individual Mappings	Localization Confidence PMID Outer Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 323 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group	BG006448 (199 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: BCAM0925 Search term: RND-8 efflux system membrane fusion protein

Human Homologs

References

Spontaneous and evolutionary changes in the antibiotic resistance of Burkholderia cenocepacia observed by global gene expression analysis. Sass A, Marchbank A, Tullis E, Lipuma JJ, Mahenthiralingam E BMC Genomics 2011 Jul 22;12:373 PubMed ID: 21781329

Differential roles of RND efflux pumps in antimicrobial drug resistance of sessile and planktonic Burkholderia cenocepacia cells. Buroni S, Matthijs N, Spadaro F, Van Acker H, Scoffone VC, Pasca MR, Riccardi G, Coenye T Antimicrob Agents Chemother 2014 Dec;58(12):7424-9 PubMed ID: 25267676

Efflux pump-mediated drug resistance in Burkholderia. Podnecky NL, Rhodes KA, Schweizer HP Front Microbiol 2015;6:305 PubMed ID: 25926825