Burkholderia cenocepacia J2315, BCAM0186 (bclA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAM0186
NCBI Locus TagQU43_RS55350
Name
bclA
Replicon chromosome 2
Genomic location 216624 - 217013 (- strand)

Cross-References

RefSeq WP_006479996.1
GI 206562056
Entrez 6927982
GI 206562056
INSDC CAR54044.1
NCBI Locus Tag QU43_RS55350
NCBI Old Locus Tag BCAM0186
RefSeq WP_006479996.1,GeneID
UniParc UPI000052D833
UniProtKB Acc B4EH87
UniProtKB ID B4EH87_BURCJ
UniRef100 UniRef100_B1K8B3
UniRef50 UniRef50_B1YYC5
UniRef90 UniRef90_B1YYC5

Product

Feature Type CDS
Coding Frame 1
Product Name
lectin
Synonyms
Evidence for Translation
Charge (pH 7) -3.29
Kyte-Doolittle Hydrophobicity Value -0.510
Molecular Weight (kDa) 13895.2
Isoelectric Point (pI) 5.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2WRA SUGAR BINDING PROTEIN 09/01/09 BclA lectin from Burkholderia cenocepacia complexed with aMan1(aMan1- 6)-3Man trisaccharide BURKHOLDERIA CENOCEPACIA 1.1 X-RAY DIFFRACTION 100.0
4AOC HYDROLASE 03/26/12 crystal structure of BC2L-A Lectin from Burkolderia cenocepacia in complex with methyl-heptoside BURKHOLDERIA CENOCEPACIA 2.7 X-RAY DIFFRACTION 100.0
2WR9 SUGAR BINDING PROTEIN 09/01/09 CRYSTAL STRUCTURE OF BURKHOLDERIA CENOCEPACIA LECTIN (BCLA) COMPLEXED WITH AMAN1-3MAN DISACCHARIDE BURKHOLDERIA CENOCEPACIA 1.75 X-RAY DIFFRACTION 100.0
2VNV SUGAR BINDING PROTEIN 02/07/08 Crystal structure of BclA lectin from burkholderia cenocepacia in complex with alpha-methyl-mannoside at 1.7 Angstrom resolution BURKHOLDERIA CENOCEPACIA 1.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 6 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG005619 (149 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAM0186
Search term: bclA
Search term: lectin

Human Homologs

References

Structural basis for mannose recognition by a lectin from opportunistic bacteria Burkholderia cenocepacia.
Lameignere E, Malinovská L, Sláviková M, Duchaud E, Mitchell EP, Varrot A, Sedo O, Imberty A, Wimmerová M
Biochem J 2008 Apr 15;411(2):307-18
PubMed ID: 18215132
Identification of functions linking quorum sensing with biofilm formation in Burkholderia cenocepacia H111.
Inhülsen S, Aguilar C, Schmid N, Suppiger A, Riedel K, Eberl L
Microbiologyopen 2012 Jun;1(2):225-42
PubMed ID: 22950027