Burkholderia cenocepacia J2315, BCAL3122 (wbiF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAL3122
NCBI Locus TagQU43_RS52340
Name
wbiF
Replicon chromosome 1
Genomic location 3413006 - 3413887 (- strand)
CommentBelongs to the O antigen biosynthesis gene cluster

Cross-References

RefSeq YP_002232233.1
GI 206561468
Entrez 6934240
GI 206561468
INSDC CAR53446.1
NCBI Locus Tag QU43_RS52340
NCBI Old Locus Tag BCAL3122
RefSeq YP_002232233.1
UniParc UPI00017B998C
UniProtKB Acc B4ECE3
UniProtKB ID B4ECE3_BURCJ
UniRef100 UniRef100_B4ECE3
UniRef50 UniRef50_B4ECE3
UniRef90 UniRef90_B4ECE3

Product

Feature Type CDS
Coding Frame 1
Product Name
glycosyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Charge (pH 7) 1.43
Kyte-Doolittle Hydrophobicity Value -0.074
Molecular Weight (kDa) 32622.7
Isoelectric Point (pI) 7.31

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database BGD
Category Lipopolysaccharide O antigen
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes wbiF mutant attenuated for virulence in agar bead model of chronic lung infection
Reference 15213146

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 281 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG006201 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAL3122
Search term: wbiF

References

Reconstitution of O-specific lipopolysaccharide expression in Burkholderia cenocepacia strain J2315, which is associated with transmissible infections in patients with cystic fibrosis.
Ortega X, Hunt TA, Loutet S, Vinion-Dubiel AD, Datta A, Choudhury B, Goldberg JB, Carlson R, Valvano MA.
J Bacteriol. 2005 Feb;187(4):1324-33.
PubMed ID: 15687196