Burkholderia cenocepacia J2315, BCAL2945 (hldA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAL2945
NCBI Locus TagQU43_RS51370
Name
hldA
Replicon chromosome 1
Genomic location 3223247 - 3224197 (- strand)

Cross-References

RefSeq YP_002232045.1
GI 206561280
Entrez 6933726
GI 206561280
INSDC CAR53246.1
NCBI Locus Tag QU43_RS51370
NCBI Old Locus Tag BCAL2945
RefSeq WP_006486931.1,GeneID
UniParc UPI00017B9732
UniProtKB Acc B4EB35
UniProtKB ID B4EB35_BURCJ
UniRef100 UniRef100_B4EB35
UniRef50 UniRef50_Q7WGU8
UniRef90 UniRef90_B4EB35

Product

Feature Type CDS
Coding Frame 1
Product Name
D-beta-D-heptose 7-phosphate kinase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -7.36
Kyte-Doolittle Hydrophobicity Value 0.092
Molecular Weight (kDa) 34004.8
Isoelectric Point (pI) 5.24

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4E84 TRANSFERASE 03/19/12 Crystal Structure of Burkholderia cenocepacia HldA BURKHOLDERIA CENOCEPACIA 2.6 X-RAY DIFFRACTION 100.0
4E8Z Transferase/Transferase Inhibitor 03/20/12 Crystal Structure of Burkholderia cenocepacia HldA in Complex with an ATP-competitive Inhibitor BURKHOLDERIA CENOCEPACIA 3.05 X-RAY DIFFRACTION 100.0
4E8W Transferase/Transferase Inhibitor 03/20/12 Crystal Structure of Burkholderia cenocepacia HldA in Complex with an ATP-competitive Inhibitor BURKHOLDERIA CENOCEPACIA 2.8654 X-RAY DIFFRACTION 100.0
4E8Y Transferase/Transferase Inhibitor 03/20/12 Crystal Structure of Burkholderia cenocepacia HldA in Complex with an ATP-competitive Inhibitor BURKHOLDERIA CENOCEPACIA 2.6 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database BGD
Category Lipopolysaccharide core region
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Rattus norvegicus
Notes hldA-/hdlD- mutant does not survive in the rat agar bead model of chronic lung infection
Reference 16513737

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 341 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000894 (653 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAL2945
Search term: hldA

References

A complete lipopolysaccharide inner core oligosaccharide is required for resistance of Burkholderia cenocepacia to antimicrobial peptides and bacterial survival in vivo.
Loutet SA, Flannagan RS, Kooi C, Sokol PA, Valvano MA
J Bacteriol 2006 Mar;188(6):2073-80
PubMed ID: 16513737
Structural-Functional Studies of Burkholderia cenocepaciad-Glycero-β-d-manno-heptose 7-Phosphate Kinase (HldA) and Characterization of Inhibitors with Antibiotic Adjuvant and Antivirulence Properties.
Lee TW, Verhey TB, Antiperovitch PA, Atamanyuk D, Desroy N, Oliveira C, Denis A, Gerusz V, Drocourt E, Loutet SA, Hamad MA, Stanetty C, Andres SN, Sugiman-Marangos S, Kosma P, Valvano MA, Moreau F, Junop MS.
J Med Chem. 2013 Jan 22. [Epub ahead of print]
PubMed ID: 23256532