Burkholderia cenocepacia J2315, BCAL1675

Gene Feature Overview

Strain	Burkholderia cenocepacia J2315 GCF_000009485.1|latest
Locus Tag	BCAL1675
NCBI Locus Tag	QU43_RS44900
Name
Replicon	chromosome 1
Genomic location	1830038 - 1833178 (+ strand)
Comment
Comment	Member of rnd-3 operon encoding Resistance-Nodulation-Cell Division (RND) efflux pump RND-3
Comment	Major RND-3 substrates are nalidixic acid, ciprofloxacin, tobramycin, chlorhexidine

Cross-References

RefSeq	WP_006487247.1
GI	206560037
Entrez	6933295
GI	206560037
INSDC	CAR51973.1
NCBI Locus Tag	QU43_RS44900
NCBI Old Locus Tag	BCAL1675
RefSeq	WP_006487247.1,GeneID
UniParc	UPI00017B9281
UniProtKB Acc	B4E8U7
UniProtKB ID	B4E8U7_BURCJ
UniRef100	UniRef100_B4E8U7
UniRef50	UniRef50_P31224
UniRef90	UniRef90_A9AIV5

Product

Feature Type	CDS
Coding Frame	1
Product Name	RND-3 efflux system transporter protein Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7)	7.17
Kyte-Doolittle Hydrophobicity Value	0.352
Molecular Weight (kDa)	111695.7
Isoelectric Point (pI)	9.14

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name	Definition	Accession	Model Type (?)	SNP	Cutoff	Bit Score	Percent Identity	CARD Version
amrB	amrB is the membrane fusion protein of the AmrAB-OprM multidrug efflux complex.	ARO:3002983	protein homolog model		Strict	0.0	86.6	1.2.1

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group	BG001444 (2569 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: BCAL1675 Search term: RND-3 efflux system transporter protein

Human Homologs

References

Assessment of three Resistance-Nodulation-Cell Division drug efflux transporters of Burkholderia cenocepacia in intrinsic antibiotic resistance. Buroni S, Pasca MR, Flannagan RS, Bazzini S, Milano A, Bertani I, Venturi V, Valvano MA, Riccardi G BMC Microbiol 2009 Sep 17;9:200 PubMed ID: 19761586

Efflux pump-mediated drug resistance in Burkholderia. Podnecky NL, Rhodes KA, Schweizer HP Front Microbiol 2015;6:305 PubMed ID: 25926825