Burkholderia cenocepacia J2315, BCAL1675

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAL1675
NCBI Locus TagQU43_RS44900
Name
Replicon chromosome 1
Genomic location 1830038 - 1833178 (+ strand)
Comment Major RND-3 substrates are nalidixic acid, ciprofloxacin, tobramycin, chlorhexidine
Comment
CommentMember of rnd-3 operon encoding Resistance-Nodulation-Cell Division (RND) efflux pump RND-3

Cross-References

RefSeq WP_006487247.1
GI 206560037
Entrez 6933295
GI 206560037
INSDC CAR51973.1
NCBI Locus Tag QU43_RS44900
NCBI Old Locus Tag BCAL1675
RefSeq WP_006487247.1,GeneID
UniParc UPI00017B9281
UniProtKB Acc B4E8U7
UniProtKB ID B4E8U7_BURCJ
UniRef100 UniRef100_B4E8U7
UniRef50 UniRef50_P31224
UniRef90 UniRef90_A9AIV5

Product

Feature Type CDS
Coding Frame 1
Product Name
RND-3 efflux system transporter protein
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 7.17
Kyte-Doolittle Hydrophobicity Value 0.352
Molecular Weight (kDa) 111695.7
Isoelectric Point (pI) 9.14

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
amrB amrB is the membrane fusion protein of the AmrAB-OprM multidrug efflux complex. ARO:3002983 protein homolog model Strict 0.0 86.6 1.2.1

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG001444 (2569 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAL1675

Human Homologs

References

Assessment of three Resistance-Nodulation-Cell Division drug efflux transporters of Burkholderia cenocepacia in intrinsic antibiotic resistance.
Buroni S, Pasca MR, Flannagan RS, Bazzini S, Milano A, Bertani I, Venturi V, Valvano MA, Riccardi G
BMC Microbiol 2009 Sep 17;9:200
PubMed ID: 19761586
Efflux pump-mediated drug resistance in Burkholderia.
Podnecky NL, Rhodes KA, Schweizer HP
Front Microbiol 2015;6:305
PubMed ID: 25926825