Burkholderia cenocepacia J2315, BCAL0348 (tssA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAL0348
NCBI Locus TagQU43_RS38275
Name
tssA
Synonym: bcsE
Replicon chromosome 1
Genomic location 375780 - 376901 (+ strand)
CommentPart of the T6SS gene cluster

Cross-References

RefSeq WP_006484398.1
GI 206558754
Entrez 6933437
GI 206558754
INSDC CAR50658.1
NCBI Locus Tag QU43_RS38275
NCBI Old Locus Tag BCAL0348
RefSeq WP_006484398.1,GeneID
UniParc UPI00017B9103
UniProtKB Acc B4E6S0
UniProtKB ID B4E6S0_BURCJ
UniRef100 UniRef100_A0A0D1D911
UniRef50 UniRef50_A1UZK2
UniRef90 UniRef90_B1JUP4

Product

Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssA
Product Name Confidence: Class 1
Synonyms putative type VI secretion system protein TssA
Evidence for Translation
Charge (pH 7) -25.59
Kyte-Doolittle Hydrophobicity Value -0.458
Molecular Weight (kDa) 41807.0
Isoelectric Point (pI) 4.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6HS5 TRANSPORT PROTEIN 09/28/18 N-terminal domain including the conserved ImpA_N region of the TssA component of the type VI secretion system from Burkholderia cenocepacia BURKHOLDERIA CENOCEPACIA H111 1.8 X-RAY DIFFRACTION 100.0
6H8E TRANSPORT PROTEIN 08/02/18 Truncated derivative of the C-terminal domain of the TssA component of the type VI secretion system from Burkholderia cenocepacia BURKHOLDERIA CENOCEPACIA H111 2.35 X-RAY DIFFRACTION 100.0
6HS6 TRANSPORT PROTEIN 09/28/18 C-terminal domain of the TssA component of the type VI secretion system from Burkholderia cenocepacia BURKHOLDERIA CENOCEPACIA H111 3.08 X-RAY DIFFRACTION 100.0
6H8F TRANSPORT PROTEIN 08/02/18 Fragment of the C-terminal domain of the TssA component of the type VI secretion system from Burkholderia cenocepacia BURKHOLDERIA CENOCEPACIA H111 1.78 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 77 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000373 (645 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAL0348
Search term: tssA

References

A novel sensor kinase-response regulator hybrid controls biofilm formation and type VI secretion system activity in Burkholderia cenocepacia.
Aubert DF, Flannagan RS, Valvano MA
Infect Immun 2008 May;76(5):1979-91
PubMed ID: 18316384
Quantification of type VI secretion system activity in macrophages infected with Burkholderia cenocepacia.
Aubert DF, Hu S, Valvano MA
Microbiology 2015 Nov;161(11):2161-73
PubMed ID: 26364149