Burkholderia mallei ATCC 23344, BMAA1523 (bopE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia mallei ATCC 23344
GCF_000011705.1|latest
Locus Tag
BMAA1523
Name
bopE
Replicon chromosome 2
Genomic location 1652361 - 1653146 (+ strand)
CommentThis gene is part of the Burkholderia mallei type III secretion system T3SS-3 gene locus.

Cross-References

RefSeq YP_106114.1
GI 53716481
NCBI Locus Tag BMAA1523
RefSeq YP_106114.1,GeneID

Product

Feature Type CDS
Coding Frame 1
Product Name
guanine nucleotide exchange factor BopE (T3SS-3)
Product Name Confidence: Class 2
Synonyms type III secretion target BopE
Evidence for Translation
Charge (pH 7) 8.14
Kyte-Doolittle Hydrophobicity Value -0.553
Molecular Weight (kDa) 28735.0
Isoelectric Point (pI) 9.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2JOK CELL INVASION, SIGNALING PROTEIN 03/14/07 NMR structure of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI NOT SOLUTION NMR 99.5
2JOL CELL INVASION, SIGNALING PROTEIN 03/14/07 Average NMR structure of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI NOT SOLUTION NMR 99.5

Virulence Evidence

Source 1
Database VFDB : VF0428
Category T3SS-3
Evidence ECO:0000250
sequence similarity evidence used in manual assertion
Host Organism
Notes
Annotation transferred from Burkholderia pseudomallei K96243
Reference 17984080

Pathogen Association Analysis

Results Analysis not done

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019825 (316 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BMAA1523
Search term: bopE

Human Homologs

References

The guanine-nucleotide-exchange factor BopE from Burkholderia pseudomallei adopts a compact version of the Salmonella SopE/SopE2 fold and undergoes a closed-to-open conformational change upon interaction with Cdc42.
Upadhyay A, Wu HL, Williams C, Field T, Galyov EE, van den Elsen JM, Bagby S
Biochem J 2008 May 1;411(3):485-93
PubMed ID: 18052936
Inactivation of Burkholderia pseudomallei bsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles.
Muangsombut V, Suparak S, Pumirat P, Damnin S, Vattanaviboon P, Thongboonkerd V, Korbsrisate S
Arch Microbiol 2008 Dec;190(6):623-31
PubMed ID: 18654761
Caspase-1-dependent and -independent cell death pathways in Burkholderia pseudomallei infection of macrophages.
Bast A, Krause K, Schmidt IH, Pudla M, Brakopp S, Hopf V, Breitbach K, Steinmetz I
PLoS Pathog 2014 Mar;10(3):e1003986
PubMed ID: 24626296
A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins.
Haque A, Chu K, Easton A, Stevens MP, Galyov EE, Atkins T, Titball R, Bancroft GJ
J Infect Dis 2006 Nov 1;194(9):1241-8
PubMed ID: 17041850
Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268
Type III Secretion in the Melioidosis Pathogen <i>Burkholderia pseudomallei</i>.
Vander Broek CW, Stevens JM
Front Cell Infect Microbiol 2017;7:255
PubMed ID: 28664152