Burkholderia thailandensis E264 ATCC 700388, BTH_I2954 (tssM-1)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia thailandensis E264 ATCC 700388
Locus Tag
NCBI Locus TagBTH_RS27260
Replicon chromosome I
Genomic location 3396298 - 3400203 (+ strand)
Transposon Mutants 4 transposon mutants in E264 ATCC 700388
CommentThis gene is part of cluster known as the type VI secretion system gene cluster tss-1 based on naming convention used for B. pseudomallei described in PMID:17660433, however unlike B. pseudomallei, the numbers following the vgrG and hcp gene match the cluster number (PMID:20865170).


RefSeq YP_443461.1
GI 83721202
Entrez 3849905
GI 83721202
INSDC AIP28572.1
NCBI Locus Tag BTH_RS27260
NCBI Old Locus Tag BTH_I2954
RefSeq WP_011402549.1
UniParc UPI00006685BC
UniProtKB Acc Q2SUD8
UniRef100 UniRef100_Q2SUD8
UniRef50 UniRef50_C4KW60
UniRef90 UniRef90_C4KW60


Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssM-1
Product Name Confidence: Class 2
Synonyms ImcF-like family protein
Evidence for Translation
Charge (pH 7) 12.44
Kyte-Doolittle Hydrophobicity Value -0.271
Molecular Weight (kDa) 142133.6
Isoelectric Point (pI) 9.49

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 101 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000378 (652 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BTH_I2954
Search term: tssM-1


Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD
PLoS Pathog 2010 Aug 26;6(8):e1001068
PubMed ID: 20865170
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434