Burkholderia thailandensis E264 ATCC 700388, BTH_II2088 (malA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia thailandensis E264 ATCC 700388
GCF_000012365.1|latest
Locus Tag
BTH_II2088
NCBI Locus TagBTH_RS10680
Name
malA
Replicon chromosome II
Genomic location 2545193 - 2553571 (+ strand)
Transposon Mutants 7 transposon mutants in E264 ATCC 700388
CommentBelongs to the malleilactone biosynthetic gene cluster

Cross-References

RefSeq YP_440276.1
GI 83718314
Entrez 3845594
GI 83718314
NCBI Locus Tag BTH_RS10680
NCBI Old Locus Tag BTH_II2088
RefSeq WP_011401503.1
UniProtKB Acc Q2T3H3
UniProtKB ID Q2T3H3_BURTA

Product

Feature Type CDS
Coding Frame 1
Product Name
polyketide synthase MalA
Product Name Confidence: Class 1
Synonyms thiotemplate mechanism natural product synthetase
Evidence for Translation
Charge (pH 7) -20.34
Kyte-Doolittle Hydrophobicity Value -0.013
Molecular Weight (kDa) 292883.3
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 636 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019774 (318 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BTH_II2088
Search term: malA

Human Homologs

Ensembl 97, assembly GRCh38.p12
fatty acid synthase [Source:HGNC Symbol;Acc:HGNC:3594]
E-value: 1.8e-54
Percent Identity: 29.8
Ensembl 97, assembly GRCh38.p12
fatty acid synthase [Source:HGNC Symbol;Acc:HGNC:3594]
E-value: 1.8e-54
Percent Identity: 29.8

References

Global Awakening of Cryptic Biosynthetic Gene Clusters in Burkholderia thailandensis.
Gupta A, Bedre R, Thapa SS, Sabrin A, Wang G, Dassanayake M, Grove A
ACS Chem Biol 2017 Dec 15;12(12):3012-3021
PubMed ID: 29087175
Malleilactone, a polyketide synthase-derived virulence factor encoded by the cryptic secondary metabolome of Burkholderia pseudomallei group pathogens.
Biggins JB, Ternei MA, Brady SF
J Am Chem Soc 2012 Aug 15;134(32):13192-5
PubMed ID: 22765305