Burkholderia thailandensis E264 ATCC 700388, BTH_II1283

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia thailandensis E264 ATCC 700388
GCF_000012365.1|latest
Locus Tag
BTH_II1283
NCBI Locus TagBTH_RS06565
Name
Replicon chromosome II
Genomic location 1527544 - 1528254 (- strand)
CommentMember of the toxoflavin-like gene cluster

Cross-References

RefSeq YP_439479.1
GI 83716101
Entrez 3845114
GI 83716101
NCBI Locus Tag BTH_RS06565
NCBI Old Locus Tag BTH_II1283
RefSeq WP_009896924.1
UniProtKB Acc Q2T5S0
UniProtKB ID Q2T5S0_BURTA

Product

Feature Type CDS
Coding Frame 1
Product Name
1,6-Didesmethyltoxoflavin N_Methyltransferase
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -2.94
Kyte-Doolittle Hydrophobicity Value -0.397
Molecular Weight (kDa) 26105.9
Isoelectric Point (pI) 6.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5UFM TRANSFERASE 01/05/17 Crystal structure of Burkholderia thailandensis 1,6-didemethyltoxoflavin-N1-methyltransferase with bound 1,6-didemethyltoxoflavin and S-adenosylhomocysteine BURKHOLDERIA THAILANDENSIS (STRAIN ATCC 700388 / DSM 13276 / CIP 106301 / E264) 1.77 X-RAY DIFFRACTION 100.0
5UFN TRANSFERASE 01/05/17 Crystal structure of Burkholderia thailandensis 1,6-didemethyltoxoflavin-N1-methyltransferase with bound S-adenosylhomocysteine BURKHOLDERIA THAILANDENSIS (STRAIN ATCC 700388 / DSM 13276 / CIP 106301 / E264) 1.39 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 51 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG018052 (324 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BTH_II1283

Human Homologs

References

Biochemical Characterization and Structural Basis of Reactivity and Regioselectivity Differences between Burkholderia thailandensis and Burkholderia glumae 1,6-Didesmethyltoxoflavin N-Methyltransferase.
Fenwick MK, Almabruk KH, Ealick SE, Begley TP, Philmus B
Biochemistry 2017 Aug 1;56(30):3934-3944
PubMed ID: 28665591