Burkholderia thailandensis E264 ATCC 700388, BTH_II0865 (tssG-5)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia thailandensis E264 ATCC 700388
GCF_000012365.1|latest
Locus Tag
BTH_II0865
NCBI Locus TagBTH_RS04490
Name
tssG-5
Replicon chromosome II
Genomic location 1015352 - 1016407 (- strand)
Transposon Mutants 2 transposon mutants in E264 ATCC 700388
CommentThis gene is part of cluster known as the type VI secretion system gene cluster tss-5 based on naming convention used for B. pseudomallei described in PMID:17660433, however unlike B. pseudomallei, the numbers following the vgrG and hcp gene match the cluster number (PMID:20865170). While other T6SSs were dispensible, T6SS-5 is required for virulence in a murine acute melioidosis model (PMID:20865170).

Cross-References

RefSeq YP_439062.1
GI 83717117
Entrez 3844528
GI 83717117
INSDC ABC35006.1
NCBI Locus Tag BTH_RS04490
NCBI Old Locus Tag BTH_II0865
RefSeq YP_439062.1
UniParc UPI0000667677
UniProtKB Acc Q2T6Y7
UniProtKB ID Q2T6Y7_BURTA
UniRef100 UniRef100_Q2T6Y7
UniRef50 UniRef50_A5PM47
UniRef90 UniRef90_A5PM47

Product

Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssG-5
Product Name Confidence: Class 2
Synonyms TssG-5
hypothetical protein
Evidence for Translation
Charge (pH 7) 14.07
Kyte-Doolittle Hydrophobicity Value -0.101
Molecular Weight (kDa) 38564.9
Isoelectric Point (pI) 10.69

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0429
Category T6SS-1
Evidence ECO:0000250
sequence similarity evidence used in manual assertion
Host Organism
Notes
Annotation transferred from Burkholderia pseudomallei K96243
Reference 17984080

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 101 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019357 (324 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BTH_II0865
Search term: tssG-5

Human Homologs

References

Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD
PLoS Pathog 2010 Aug 26;6(8):e1001068
PubMed ID: 20865170
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434