Burkholderia pseudomallei K96243, BPSS1545 (bsaO)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS1545
Name
bsaO
Synonym: sctC
Replicon chromosome 2
Genomic location 2098689 - 2100551 (- strand)
CommentThis gene is part of the type III secretion system T3SS-3 (bsa) gene locus.

Cross-References

RefSeq YP_111551.1
GI 53722566
Entrez 3096571
GI 53722566
INSDC CAH39018.1
NCBI Locus Tag BPSS1545
RefSeq YP_111551.1,GeneID
UniParc UPI000043BD2B
UniProtKB Acc Q63K21
UniProtKB ID Q63K21_BURPS
UniRef100 UniRef100_A0A069BGZ0
UniRef50 UniRef50_A3MCF5
UniRef90 UniRef90_A3MCF5

Product

Feature Type CDS
Coding Frame 1
Product Name
type III secretion system outer membrane ring protein / secretin BsaO (T3SS-3)
Product Name Confidence: Class 2
Synonyms Type III secretion system protein
Evidence for Translation
Detected in B. pseudomallei strain DD503 using trypsin shaving and mass spectrometry (PMID:21391724). Found in at least two preparations.
Charge (pH 7) -6.57
Kyte-Doolittle Hydrophobicity Value -0.148
Molecular Weight (kDa) 66135.8
Isoelectric Point (pI) 5.86

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Additional evidence for subcellular localization
Detected in Triton-insoluble fraction of lysozyme-treated B. pseudomallei DD503 cells using trypsin shaving and mass spectrometry (PMID:21391724).

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0428
Category T3SS-3
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Notes
Reference 17984080

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 221 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019850 (324 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1545
Search term: bsaO

Human Homologs

References

Unraveling type III secretion systems in the highly versatile Burkholderia pseudomallei.
Sun GW, Gan YH
Trends Microbiol 2010 Dec;18(12):561-8
PubMed ID: 20951592
Type III Secretion in the Melioidosis Pathogen <i>Burkholderia pseudomallei</i>.
Vander Broek CW, Stevens JM
Front Cell Infect Microbiol 2017;7:255
PubMed ID: 28664152
Composition, formation, and regulation of the cytosolic c-ring, a dynamic component of the type III secretion injectisome.
Diepold A, Kudryashev M, Delalez NJ, Berry RM, Armitage JP
PLoS Biol 2015 Jan;13(1):e1002039
PubMed ID: 25591178