Burkholderia pseudomallei K96243, BPSS1542 (bsaR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS1542
Name
bsaR
Replicon chromosome 2
Genomic location 2095059 - 2095466 (- strand)
CommentThis gene is part of the type III secretion system T3SS-3 (bsa) gene locus. BsaR is homologous to the Salmonella chaperone InvB which has been shown to be required for secretion of the effector proteins SopE and SopA (PMID:14645290). BsaR has been predicted to be the chaperone for BopE using a computational screen for chaperones in B. pseudomallei K96243 (PMID:16164564) but this has not been validated yet.

Cross-References

RefSeq YP_111548.1
GI 53722563
Entrez 3096685
GI 53722563
INSDC CAH39015.1
NCBI Locus Tag BPSS1542
RefSeq YP_111548.1,GeneID
UniParc UPI000043BD28
UniProtKB Acc Q63K24
UniProtKB ID Q63K24_BURPS
UniRef100 UniRef100_A0A069BE17
UniRef50 UniRef50_Q2T721
UniRef90 UniRef90_Q2T721

Product

Feature Type CDS
Coding Frame 1
Product Name
chaperone protein BsaR (T3SS-3)
Product Name Confidence: Class 2
Synonyms surface presentation of antigens protein
Evidence for Translation
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (14.1 kDa, pI 4.49, PMID:21960737)
Charge (pH 7) -10.23
Kyte-Doolittle Hydrophobicity Value 0.182
Molecular Weight (kDa) 14837.8
Isoelectric Point (pI) 4.36

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Extracellular Class 1
quantitative mass spectrometry evidence
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0428
Category T3SS-3
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Notes
Reference 17984080

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 11 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019846 (320 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1542
Search term: bsaR

References

Unraveling type III secretion systems in the highly versatile Burkholderia pseudomallei.
Sun GW, Gan YH
Trends Microbiol 2010 Dec;18(12):561-8
PubMed ID: 20951592
Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268
Type III Secretion in the Melioidosis Pathogen <i>Burkholderia pseudomallei</i>.
Vander Broek CW, Stevens JM
Front Cell Infect Microbiol 2017;7:255
PubMed ID: 28664152
A genome-wide screen identifies a Bordetella type III secretion effector and candidate effectors in other species.
Panina EM, Mattoo S, Griffith N, Kozak NA, Yuk MH, Miller JF
Mol Microbiol 2005 Oct;58(1):267-79
PubMed ID: 16164564
InvB is a type III secretion-associated chaperone for the Salmonella enterica effector protein SopE.
Lee SH, Galán JE
J Bacteriol 2003 Dec;185(24):7279-84
PubMed ID: 14645290