Burkholderia pseudomallei K96243, BPSS1539 (bsaU)

Gene Feature Overview

Strain	Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1 GCF_000011545.1|latest
Locus Tag	BPSS1539
Name	bsaU Synonym: sctP
Replicon	chromosome 2
Genomic location	2092039 - 2093316 (- strand)
Comment	This gene is part of the type III secretion system T3SS-3 (bsa) gene locus. BsaU is homologous to InvJ from Salmonella which is the molecular ruler which determines the length of the T3SS needle. (PMID: 10944190).

Cross-References

RefSeq	YP_111545.1
GI	53722560
Entrez	3096285
GI	53722560
INSDC	CAH39012.1
NCBI Locus Tag	BPSS1539
RefSeq	YP_111545.1,GeneID
UniParc	UPI000043BD25
UniProtKB Acc	Q63K27
UniProtKB ID	Q63K27_BURPS
UniRef100	UniRef100_Q63K27
UniRef50	UniRef50_A3MCG1
UniRef90	UniRef90_A3NLE2

Product

Feature Type	CDS
Coding Frame	1
Product Name	needle length control protein BsaU (T3SS-3) Product Name Confidence: Class 2
Synonyms	hypothetical protein
Evidence for Translation	Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (17.3 kDa, pI 4.83, PMID:21960737)
Charge (pH 7)	10.33
Kyte-Doolittle Hydrophobicity Value	-0.631
Molecular Weight (kDa)	43159.7
Isoelectric Point (pI)	10.66

Subcellular localization

Individual Mappings	Localization Confidence PMID Unknown Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database	VFDB : VF0428
Category	T3SS-3
Evidence	ECO:0000314 direct assay evidence used in manual assertion
Host Organism
Notes
Reference	17984080
Source 2
Database	Victors : 1985
Category
Evidence	ECO:0000015 mutant phenotype evidence
Host Organism	Mus musculus
Notes	BPSS1539 mutant is attenuated in mice
Reference	16714590

Pathogen Association Analysis

Results

Pathogenic Found only in pathogenic strains Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Burkholderia Ortholog Group	BG019843 (305 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: BPSS1539 Search term: bsaU Search term: needle length control protein BsaU (T3SS-3)

Human Homologs

References

Unraveling type III secretion systems in the highly versatile Burkholderia pseudomallei. Sun GW, Gan YH Trends Microbiol 2010 Dec;18(12):561-8 PubMed ID: 20951592

Type III Secretion in the Melioidosis Pathogen <i>Burkholderia pseudomallei</i>. Vander Broek CW, Stevens JM Front Cell Infect Microbiol 2017;7:255 PubMed ID: 28664152

Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system. Kubori T, Sukhan A, Aizawa SI, Galán JE Proc Natl Acad Sci U S A 2000 Aug 29;97(18):10225-30 PubMed ID: 10944190

Identification of Burkholderia pseudomallei genes required for the intracellular life cycle and in vivo virulence. Pilatz S, Breitbach K, Hein N, Fehlhaber B, Schulze J, Brenneke B, Eberl L, Steinmetz I Infect Immun 2006 Jun;74(6):3576-86 PubMed ID: 16714590