Burkholderia pseudomallei K96243, BPSS1533 (bicA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
Locus Tag
Replicon chromosome 2
Genomic location 2087441 - 2087956 (- strand)
CommentThis gene is part of the type III secretion system T3SS-3 (bsa) gene locus. The B. pseudomallei bicA gene is able to partially complement a Salmonella sicA mutant, supporting a homologous function as chaperone (PMID:27997726).


RefSeq YP_111539.1
GI 53722554
Entrez 3096042
GI 53722554
INSDC CAH39006.1
NCBI Locus Tag BPSS1533
RefSeq YP_111539.1,GeneID
UniParc UPI000043BD1F
UniProtKB Acc Q63K33
UniRef100 UniRef100_A0A069B9Y9
UniRef50 UniRef50_Q7NUS7
UniRef90 UniRef90_I1WUC1


Feature Type CDS
Coding Frame 1
Product Name
type III secretion chaperone protein BicA (T3SS-3)
Product Name Confidence: Class 2
Synonyms surface presentation of antigens protein
Evidence for Translation
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (16.0 kDa, pI 6.00, PMID:21960737)
Charge (pH 7) -7.93
Kyte-Doolittle Hydrophobicity Value -0.227
Molecular Weight (kDa) 19198.5
Isoelectric Point (pI) 4.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0428
Category T3SS-3
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Reference 17984080

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 28 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019836 (323 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1533
Search term: bicA


Unraveling type III secretion systems in the highly versatile Burkholderia pseudomallei.
Sun GW, Gan YH
Trends Microbiol 2010 Dec;18(12):561-8
PubMed ID: 20951592
Type III secretion chaperone-dependent regulation: activation of virulence genes by SicA and InvF in Salmonella typhimurium.
Darwin KH, Miller VL
EMBO J 2001 Apr 17;20(8):1850-62
PubMed ID: 11296219
Type III Secretion in the Melioidosis Pathogen <i>Burkholderia pseudomallei</i>.
Vander Broek CW, Stevens JM
Front Cell Infect Microbiol 2017;7:255
PubMed ID: 28664152
Identification of a regulatory cascade controlling Type III Secretion System 3 gene expression in Burkholderia pseudomallei.
Sun GW, Chen Y, Liu Y, Tan GY, Ong C, Tan P, Gan YH
Mol Microbiol 2010 May;76(3):677-89
PubMed ID: 20345664
Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon.
Chen Y, Schröder I, French CT, Jaroszewicz A, Yee XJ, Teh BE, Toesca IJ, Miller JF, Gan YH
BMC Microbiol 2014 Aug 1;14:206
PubMed ID: 25085508
Functional relatedness in the Inv/Mxi-Spa type III secretion system family.
Klein JA, Dave BM, Raphenya AR, McArthur AG, Knodler LA
Mol Microbiol 2017 Mar;103(6):973-991
PubMed ID: 27997726