Burkholderia pseudomallei K96243, BPSS1356

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS1356
Name
Replicon chromosome 2
Genomic location 1853417 - 1856794 (+ strand)

Cross-References

RefSeq YP_111366.1
GI 53722381
Entrez 3096459
GI 53722381
INSDC CAH38827.1
NCBI Locus Tag BPSS1356
RefSeq YP_111366.1,GeneID
UniParc UPI000043BC7C
UniProtKB Acc Q63KK6
UniProtKB ID Q63KK6_BURPS
UniRef100 UniRef100_A0A069B9X4
UniRef50 UniRef50_A1UVS7
UniRef90 UniRef90_A1UVS7

Product

Feature Type CDS
Coding Frame 1
Product Name
regulatory protein
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Detected in B. pseudomallei strain DD503 using trypsin shaving and mass spectrometry (PMID:21391724).
Charge (pH 7) -15.06
Kyte-Doolittle Hydrophobicity Value -0.203
Molecular Weight (kDa) 124133.0
Isoelectric Point (pI) 6.22

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
quantitative mass spectrometry evidence
Cytoplasmic Class 3
Additional evidence for subcellular localization
Detected in Triton-insoluble fraction of lysozyme-treated B. pseudomallei DD503 cells using trypsin shaving and mass spectrometry (PMID:21391724).

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5IKY HYDROLASE,LYASE 03/04/16 Apo structure of Obc1, a bifunctional enzyme for quorum sensing-dependent oxalogenesis BURKHOLDERIA THAILANDENSIS (STRAIN E264 / ATCC 700388 / DSM 13276 / CIP 106301) 2.5 X-RAY DIFFRACTION 95.0
5IKZ HYDROLASE,LYASE 03/04/16 Glycerol bound structure of Obc1, a bifunctional enzyme for quorum sensing-dependent oxalogenesis BURKHOLDERIA THAILANDENSIS (STRAIN E264 / ATCC 700388 / DSM 13276 / CIP 106301) 2.8 X-RAY DIFFRACTION 95.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 23 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019464 (440 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1356

Human Homologs

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268
The multiple roles of hypothetical gene BPSS1356 in Burkholderia pseudomallei.
Yam H, Rahim AA, Mohamad S, Mahadi NM, Manaf UA, Shu-Chien AC, Najimudin N
PLoS One 2014;9(6):e99218
PubMed ID: 24927285
Validation of a Burkholderia pseudomallei hypothetical protein and determination of its translational start codon using chromosomal integration of His-Tag coding sequence.
Yam H, Abdul Rahim A, Gim Luan O, Samian R, Abdul Manaf U, Mohamad S, Najimudin N
Protein J 2012 Mar;31(3):246-9
PubMed ID: 22354666