Burkholderia pseudomallei K96243, BPSS0518 (tssF-6)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS0518
Name
tssF-6
Synonym: hcp2
Synonym: tssD-4
Replicon chromosome 2
Genomic location 705288 - 705815 (+ strand)
CommentGene is part of a cluster known as the type VI secretion system gene cluster tss-4 used in much of the early P. pseudomallei literature (PMID:17660433) but later referred to as T6SS-2 cluster later (PMID:22007185)
CommentGene is part of a cluster known as the type VI secretion system gene cluster tss-6 used in much of the early P. pseudomallei literature (PMID:17660433) but later referred to as T6SS-3 cluster later (PMID:22007185)

Cross-References

RefSeq YP_110539.1
GI 53721554
Entrez 3096785
GI 53721554
INSDC CAH37975.1
NCBI Locus Tag BPSS0518
RefSeq YP_110539.1,GeneID
UniParc UPI000043B951
UniProtKB Acc Q63MY0
UniProtKB ID Q63MY0_BURPS
UniRef100 UniRef100_A0A069B759
UniRef50 UniRef50_A1UWJ9
UniRef90 UniRef90_A1UWJ9

Product

Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssF-6
Product Name Confidence: Class 2
Synonyms hypothetical protein
type VI secretion system protein TssD-4
Evidence for Translation
Detected in B. pseudomallei strain DD503 using trypsin shaving and mass spectrometry (PMID:21391724). Found in at least two preparations.
Charge (pH 7) -3.59
Kyte-Doolittle Hydrophobicity Value -0.331
Molecular Weight (kDa) 19247.6
Isoelectric Point (pI) 4.98

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization
Detected in Triton-insoluble fraction of lysozyme-treated B. pseudomallei DD503 cells using trypsin shaving and mass spectrometry (PMID:21391724).

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 57 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019180 (327 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS0518
Search term: tssF-6

Human Homologs

References

In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434