Burkholderia pseudomallei K96243, BPSL3221 (rpoB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL3221
Name
rpoB
Replicon chromosome 1
Genomic location 3820448 - 3824554 (- strand)

Cross-References

RefSeq YP_109815.1
GI 53720829
Entrez 3092566
GI 53720829
INSDC CAH37232.1
NCBI Locus Tag BPSL3221
RefSeq YP_109815.1,GeneID
UniParc UPI000043B686
UniProtKB Acc Q63Q03
UniProtKB ID RPOB_BURPS
UniRef100 UniRef100_A3MRU5
UniRef50 UniRef50_O52271
UniRef90 UniRef90_A3MRU5

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA-directed RNA polymerase subunit beta
Synonyms
Evidence for Translation
Charge (pH 7) -26.24
Kyte-Doolittle Hydrophobicity Value -0.337
Molecular Weight (kDa) 153149.3
Isoelectric Point (pI) 5.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Extracellular Class 1
quantitative mass spectrometry evidence
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 667 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000250 (653 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL3221
Search term: rpoB

Human Homologs

Ensembl 97, assembly GRCh38.p12
RNA polymerase III subunit B [Source:HGNC Symbol;Acc:HGNC:30348]
E-value: 1.2e-27
Percent Identity: 30.5
Ensembl 97, assembly GRCh38.p12
RNA polymerase III subunit B [Source:HGNC Symbol;Acc:HGNC:30348]
E-value: 1.2e-27
Percent Identity: 30.5

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268