Burkholderia pseudomallei K96243, BPSL3105 (tssD-1)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
Locus Tag
Synonym: hcp6
Replicon chromosome 1
Genomic location 3710641 - 3711144 (- strand)
CommentGene is part of a cluster known as the type VI secretion system gene cluster tss-1 used in much of the early P. pseudomallei literature (PMID:17660433) but later referred to as T6SS-1 cluster later (PMID:22007185)


RefSeq YP_109700.1
GI 53720714
Entrez 3093075
GI 53720714
INSDC CAH37116.1
NCBI Locus Tag BPSL3105
RefSeq YP_109700.1,GeneID
UniParc UPI000043B61B
UniProtKB Acc Q63QB8
UniRef100 UniRef100_I1WF16
UniRef50 UniRef50_D8N062
UniRef90 UniRef90_I1WF16


Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssD-1
Product Name Confidence: Class 2
Synonyms hypothetical protein
Evidence for Translation
Detected in B. pseudomallei strain DD503 using trypsin shaving and mass spectrometry (PMID:21391724). Found in at least two preparations.
Charge (pH 7) -0.12
Kyte-Doolittle Hydrophobicity Value -0.472
Molecular Weight (kDa) 18334.5
Isoelectric Point (pI) 6.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
quantitative mass spectrometry evidence
Additional evidence for subcellular localization
Detected in Triton-insoluble fraction of lysozyme-treated B. pseudomallei DD503 cells using trypsin shaving and mass spectrometry (PMID:21391724).

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 81 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000368 (644 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BPSL3105
Search term: tssD-1


Quantitative Proteomic Analysis of Burkholderia pseudomallei Bsa Type III Secretion System Effectors Using Hypersecreting Mutants.
Vander Broek CW(1), Chalmers KJ(2), Stevens MP(1), Stevens JM(3).
Mol Cell Proteomics. 2015 Apr;14(4):905-16.
PubMed ID: 25635268
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS.
Microbiology. 2007 Aug;153(Pt 8):2689-99.
PubMed ID: 17660433
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA(1), Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D.
Mol Microbiol. 2007 Jun;64(6):1466-85.
PubMed ID: 17555434