Burkholderia pseudomallei K96243, BPSL2795 (gmhA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL2795
Name
gmhA
Synonym: Sedoheptulose 7-phosphate isomerase
Replicon chromosome 1
Genomic location 3342168 - 3342761 (- strand)

Cross-References

RefSeq YP_109390.1
GI 53720404
Entrez 3092932
GI 53720404
NCBI Locus Tag BPSL2795
RefSeq YP_109390.1,GeneID
UniProtKB Acc Q93UJ2
UniProtKB ID GMHA_BURPS

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphoheptose isomerase
Synonyms
Evidence for Translation
Charge (pH 7) -3.17
Kyte-Doolittle Hydrophobicity Value 0.077
Molecular Weight (kDa) 20812.6
Isoelectric Point (pI) 5.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5LU5 ISOMERASE 09/08/16 A quantum half-site enzyme BURKHOLDERIA PSEUDOMALLEI 1.55 X-RAY DIFFRACTION 99.5
5LTZ ISOMERASE 09/07/16 GmhA_mutant Q175E BURKHOLDERIA PSEUDOMALLEI K96243 1.67 X-RAY DIFFRACTION 99.5
2X3Y ISOMERASE 01/28/10 Crystal structure of GmhA from Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI 2.4 X-RAY DIFFRACTION 100.0
2XBL ISOMERASE 04/13/10 Crystal structure of GmhA from Burkholderia pseudomallei in complex with product BURKHOLDERIA PSEUDOMALLEI 1.62 X-RAY DIFFRACTION 100.0
5LU7 ISOMERASE 09/08/16 Heptose isomerase GmhA mutant - D61A BURKHOLDERIA PSEUDOMALLEI K96243 1.92 X-RAY DIFFRACTION 99.5
5LU6 ISOMERASE 09/08/16 Heptose isomerase mutant - H64Q BURKHOLDERIA PSEUDOMALLEI 1.67 X-RAY DIFFRACTION 99.5

Virulence Evidence

Source 1
Database VFDB : VF0436
Category Type I O-polysaccharide
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Notes
Reference 17984080

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 358 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG020186 (332 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL2795
Search term: gmhA

Human Homologs

References

A preliminary X-ray study of sedoheptulose-7-phosphate isomerase from Burkholderia pseudomallei.
Kim MS, Shin DH
Acta Crystallogr Sect F Struct Biol Cryst Commun 2009 Nov 1;65(Pt 11):1110-2
PubMed ID: 19923728
The structure of sedoheptulose-7-phosphate isomerase from Burkholderia pseudomallei reveals a zinc binding site at the heart of the active site.
Harmer NJ
J Mol Biol 2010 Jul 16;400(3):379-92
PubMed ID: 20447408