Burkholderia pseudomallei K96243, BPSL1524 (purA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL1524
Name
purA
Replicon chromosome 1
Genomic location 1763184 - 1764530 (+ strand)

Cross-References

RefSeq YP_108144.1
GI 53719158
Entrez 3093829
GI 53719158
INSDC CAH35525.1
NCBI Locus Tag BPSL1524
RefSeq YP_108144.1,GeneID
UniParc UPI000043B035
UniProtKB Acc Q63US2
UniProtKB ID PURA_BURPS
UniRef100 UniRef100_A3NVV9
UniRef50 UniRef50_P45283
UniRef90 UniRef90_Q2SWD3

Product

Feature Type CDS
Coding Frame 1
Product Name
adenylosuccinate synthetase
Synonyms
Evidence for Translation
Charge (pH 7) -5.24
Kyte-Doolittle Hydrophobicity Value -0.127
Molecular Weight (kDa) 48330.5
Isoelectric Point (pI) 5.92

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
quantitative mass spectrometry evidence
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3UE9 LIGASE 10/28/11 Crystal structure of Adenylosuccinate synthetase (AMPSase) (purA) from Burkholderia thailandensis BURKHOLDERIA THAILANDENSIS 1.95 X-RAY DIFFRACTION 98.4

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 640 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG001638 (652 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL1524
Search term: purA

Human Homologs

Ensembl 97, assembly GRCh38.p12
adenylosuccinate synthase like 1 [Source:HGNC Symbol;Acc:HGNC:20093]
E-value: 2.5e-91
Percent Identity: 42.0

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268