Burkholderia pseudomallei K96243, BPSL1021 (ppa)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL1021
Name
ppa
Replicon chromosome 1
Genomic location 1190916 - 1191443 (+ strand)

Cross-References

RefSeq YP_107649.1
GI 53718663
Entrez 3094000
GI 53718663
INSDC CAH35017.1
NCBI Locus Tag BPSL1021
RefSeq YP_107649.1,GeneID
UniParc UPI000043AE47
UniProtKB Acc Q63W67
UniProtKB ID Q63W67_BURPS
UniRef100 UniRef100_A0A095QE80
UniRef50 UniRef50_P0A7B0
UniRef90 UniRef90_Q143A4

Product

Feature Type CDS
Coding Frame 1
Product Name
inorganic pyrophosphatase
Synonyms
Evidence for Translation
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (21.0 kDa, pI 5.25, PMID:21960737)
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (20.8 kDa, pI 5.02, PMID:21960737)
Charge (pH 7) -3.32
Kyte-Doolittle Hydrophobicity Value -0.071
Molecular Weight (kDa) 19161.0
Isoelectric Point (pI) 5.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Extracellular Class 1
quantitative mass spectrometry evidence
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3EJ0 HYDROLASE 09/17/08 Crystal structure of inorganic pyrophosphatase from burkholderia pseudomallei with bound N-(pyridin-3-ylmethyl) aniline, H32 crystal form BURKHOLDERIA PSEUDOMALLEI 1710B 1.96 X-RAY DIFFRACTION 100.0
3EIY HYDROLASE 09/17/08 Crystal structure of inorganic pyrophosphatase from burkholderia pseudomallei with bound pyrophosphate BURKHOLDERIA PSEUDOMALLEI 1710B 2.1 X-RAY DIFFRACTION 100.0
3EIZ HYDROLASE 09/17/08 Crystal structure of inorganic pyrophosphatase from burkholderia pseudomallei, H32 crystal form BURKHOLDERIA PSEUDOMALLEI 1710B 1.88 X-RAY DIFFRACTION 100.0
3D63 HYDROLASE 05/18/08 Crystal structure of inorganic pyrophosphatase from Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI 2.3 X-RAY DIFFRACTION 100.0
3EJ2 HYDROLASE 09/17/08 Crystal structure of inorganic pyrophosphatase from burkholderia pseudomallei with bound 5-amino-1-(4-chlorophenyl)-1h-pyrazole-4-carbonitrile, H32 crystal form BURKHOLDERIA PSEUDOMALLEI 1710B 2.12 X-RAY DIFFRACTION 100.0
3GVF HYDROLASE 03/30/09 1.7 Angstrom crystal structure of inorganic pyrophosphatase from burkholderia pseudomallei bound with phosphate BURKHOLDERIA PSEUDOMALLEI 1710B 1.75 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N-(pyridin-3-ylmethyl)aniline DB06851 DrugBank 5.1.5 Q3JUV5 Inorganic pyrophosphatase 100.0 175 1.1e-97
5-amino-1-(4-chlorophenyl)-1H-pyrazole-4-carbonitrile DB07291 DrugBank 5.1.5 Q3JUV5 Inorganic pyrophosphatase 100.0 175 1.1e-97

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 483 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG002287 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL1021
Search term: ppa

Human Homologs

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268