Burkholderia cenocepacia K56-2, WQ49_RS09445

Cytoplasmic Membrane
Outer Membrane

Gene Feature Overview

Strain Burkholderia cenocepacia K56-2
Locus Tag
NCBI Old Locus TagWQ49_09455
Synonym: PaaZ-ECH
Replicon scf7180000000050
Genomic location 282343 - 282801 (- strand)
CommentIn E. coli, paaZ encodes a bifunctional protein consisting of an N-terminal NADP-dependent aldehyde dehydrogenase domain and a C-terminal MaoC-like (R)-specific enoyl-CoA hydratase domain. In Burkholderia genomes, a gene incorrectly annotated as paaZ (e.g WQ49_RS11500) encodes only one of the two domains found in E.coli paaZ (the domain with semialdehyde dehydrogenase activity) and so is renamed PacL (PMID:21296885) but was also referred to as PaaZ-ALDH in the manuscript. In the same study , a separate A. aromaticum, protein (UniProtKB:Q5P232) with the (R)-hydratase activity was annotated as Ech-Aa and the Burkholderia ortholog was annotated as PaaZ-ECH.


RefSeq WP_006483300.1
GI 493529284
NCBI Locus Tag WQ49_RS09445
NCBI Old Locus Tag WQ49_09455
RefSeq WP_006483300.1


Feature Type CDS
Coding Frame 1
Product Name
putative (R)-specific oxepin-CoA hydratase
Product Name Confidence: Class 2
Synonyms acyl dehydratase
Evidence for Translation
Charge (pH 7) -2.63
Kyte-Doolittle Hydrophobicity Value -0.153
Molecular Weight (kDa) 16901.1
Isoelectric Point (pI) 6.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG005317 (313 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: WQ49_RS09445

Human Homologs


Studies on the mechanism of ring hydrolysis in phenylacetate degradation: a metabolic branching point.
Teufel R, Gantert C, Voss M, Eisenreich W, Haehnel W, Fuchs G
J Biol Chem 2011 Apr 1;286(13):11021-34
PubMed ID: 21296885