Burkholderia ambifaria AMMD, BAMB_RS27655 (bceC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia ambifaria AMMD - Assembly GCF_000203915.1
GCF_000203915.1|latest
Locus Tag
BAMB_RS27655
NCBI Old Locus TagBamb_5547
Name
bceC
Replicon chromosome 2
Genomic location 2619455 - 2620867 (+ strand)
CommentMember of the bce-I gene cluster

Cross-References

RefSeq WP_011660457.1
GI 499979739
NCBI Locus Tag BAMB_RS27655
NCBI Old Locus Tag Bamb_5547
RefSeq WP_011660457.1

Product

Feature Type CDS
Coding Frame NA
Product Name
UDP-Glucose Dehydrogenase BceC
Product Name Confidence: Class 2
Synonyms UDP-glucose 6-dehydrogenase
Evidence for Translation
Charge (pH 7) -8.20
Kyte-Doolittle Hydrophobicity Value -0.178
Molecular Weight (kDa) 51310.6
Isoelectric Point (pI) 5.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2Y0D OXIDOREDUCTASE 12/02/10 BceC mutation Y10K BURKHOLDERIA CEPACIA 2.8 X-RAY DIFFRACTION 96.6
2Y0C OXIDOREDUCTASE 12/02/10 BceC mutation Y10S BURKHOLDERIA CEPACIA 1.75 X-RAY DIFFRACTION 96.6
2Y0E OXIDOREDUCTASE 12/02/10 BceC and the final step of UGDs reaction BURKHOLDERIA CEPACIA 1.75 X-RAY DIFFRACTION 96.8

Pathogen Association Analysis

Results Analysis not done

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000893 (1284 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BAMB_RS27655
Search term: bceC

Human Homologs

Ensembl 97, assembly GRCh38.p12
UDP-glucose 6-dehydrogenase [Source:HGNC Symbol;Acc:HGNC:12525]
E-value: 1.4e-65
Percent Identity: 35.8
Ensembl 97, assembly GRCh38.p12
UDP-glucose 6-dehydrogenase [Source:HGNC Symbol;Acc:HGNC:12525]
E-value: 1.4e-65
Percent Identity: 35.8

References

Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions.
Ferreira AS, Leitão JH, Silva IN, Pinheiro PF, Sousa SA, Ramos CG, Moreira LM
Appl Environ Microbiol 2010 Jan;76(2):441-50
PubMed ID: 19948863
Structure of Burkholderia cepacia UDP-glucose dehydrogenase (UGD) BceC and role of Tyr10 in final hydrolysis of UGD thioester intermediate.
Rocha J, Popescu AO, Borges P, Mil-Homens D, Moreira LM, Sá-Correia I, Fialho AM, Frazão C
J Bacteriol 2011 Aug;193(15):3978-87
PubMed ID: 21602353
Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose dehydrogenase from Burkholderia cepacia IST408.
Rocha J, Popescu AO, Sá-Correia I, Fialho AM, Frazão C
Acta Crystallogr Sect F Struct Biol Cryst Commun 2010 Mar 1;66(Pt 3):269-71
PubMed ID: 20208157