Burkholderia cenocepacia K56-2Valvano, BURCENK562V_RS06245 (dfsA)

Cytoplasmic Membrane
Outer Membrane

Gene Feature Overview

Strain Burkholderia cenocepacia K56-2Valvano
Locus Tag
NCBI Old Locus TagBURCENK562V_C2565
Synonym: rpfF
Replicon ctg7180000002940B
Genomic location 69118 - 69981 (- strand)


RefSeq WP_006488766.1
GI 493534800
EC Number
GI 493534800
NCBI Locus Tag BURCENK562V_RS06245
NCBI Old Locus Tag BURCENK562V_C2565
RefSeq WP_006488766.1


Feature Type CDS
Coding Frame 1
Product Name
diffusible factor synthase A
Product Name Confidence: Class 2
Synonyms enoyl-CoA hydratase
Evidence for Translation
Charge (pH 7) -3.68
Kyte-Doolittle Hydrophobicity Value 0.006
Molecular Weight (kDa) 32000.4
Isoelectric Point (pI) 5.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5FUS LYASE 01/29/16 Crystal structure of B. cenocepacia DfsA BURKHOLDERIA CENOCEPACIA 1.87 X-RAY DIFFRACTION 100.0
6DGN UNKNOWN FUNCTION 05/17/18 Cronobacter turicensis BDSF synthase RpfF in complex with the RpfR quorum-sensing receptor FI domain BURKHOLDERIA CENOCEPACIA PC184; CRONOBACTER TURICENSIS (STRAIN DSM 18703 / LMG 23827 / Z3032) 1.999 X-RAY DIFFRACTION 99.3

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG004647 (313 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: dfsA


A novel DSF-like signal from Burkholderia cenocepacia interferes with Candida albicans morphological transition.
Boon C, Deng Y, Wang LH, He Y, Xu JL, Fan Y, Pan SQ, Zhang LH
ISME J 2008 Jan;2(1):27-36
PubMed ID: 18049456
Quorum sensing systems influence Burkholderia cenocepacia virulence.
Subramoni S, Sokol PA
Future Microbiol 2012 Dec;7(12):1373-87
PubMed ID: 23231487
The host plant metabolite glucose is the precursor of diffusible signal factor (DSF) family signals in Xanthomonas campestris.
Deng Y, Liu X, Wu J, Lee J, Chen S, Cheng Y, Zhang C, Zhang LH
Appl Environ Microbiol 2015 Apr;81(8):2861-8
PubMed ID: 25681189
The DSF type quorum sensing signalling system RpfF/R regulates diverse phenotypes in the opportunistic pathogen Cronobacter.
Suppiger A, Eshwar AK, Stephan R, Kaever V, Eberl L, Lehner A
Sci Rep 2016 Jan 4;6:18753
PubMed ID: 26725701
The Burkholderia cenocepacia BDSF quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities.
Bi H, Christensen QH, Feng Y, Wang H, Cronan JE
Mol Microbiol 2012 Feb;83(4):840-55
PubMed ID: 22221091