Burkholderia cenocepacia K56-2Valvano, BURCENK562V_RS22210 (arnT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Burkholderia cenocepacia K56-2Valvano
GCF_000333155.2|latest
Locus Tag
BURCENK562V_RS22210
NCBI Old Locus TagBURCENK562V_C1274
Name
arnT
Replicon ctg7180000002937
Genomic location 434710 - 436386 (- strand)
CommentIn PMID:27597847: arnT, arnB, arnC, arnA1, arnA2 and BURCENK562V_RS22180 ortholog BCAL1935 located in the arn gene cluster harbored significantly low frequencies of transposon insertions (TraDIS). They were therefore categorized as essential by the authors.

Cross-References

RefSeq WP_006482861.1
GI 493528844
EC Number 2.4.1.109
GI 493528844
NCBI Locus Tag BURCENK562V_RS22210
NCBI Old Locus Tag BURCENK562V_C1274
RefSeq WP_006482861.1

Product

Feature Type CDS
Coding Frame 1
Product Name
4-amino-4-deoxy-L-arabinose transferase ArnT
Product Name Confidence: Class 2
Synonyms 4-amino-4-deoxy-L-arabinose transferase
Evidence for Translation
Charge (pH 7) 18.09
Kyte-Doolittle Hydrophobicity Value 0.493
Molecular Weight (kDa) 62686.3
Isoelectric Point (pI) 10.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
direct assay evidence used in manual assertion
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG001691 (653 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: arnT

Human Homologs

References

PubMed ID: 22742453
ArnT proteins that catalyze the glycosylation of lipopolysaccharide share common features with bacterial N-oligosaccharyltransferases.
Tavares-Carreón F, Fathy Mohamed Y, Andrade A, Valvano MA
Glycobiology 2016 Mar;26(3):286-300
PubMed ID: 26515403
Burkholderia cenocepacia and Salmonella enterica ArnT proteins that transfer 4-amino-4-deoxy-l-arabinose to lipopolysaccharide share membrane topology and functional amino acids.
Tavares-Carreón F, Patel KB, Valvano MA
Sci Rep 2015 Jun 1;5:10773
PubMed ID: 26030265
An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor.
Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR
J Biol Chem 2001 Nov 16;276(46):43122-31
PubMed ID: 11535604
A putative gene cluster for aminoarabinose biosynthesis is essential for Burkholderia cenocepacia viability.
Ortega XP, Cardona ST, Brown AR, Loutet SA, Flannagan RS, Campopiano DJ, Govan JR, Valvano MA
J Bacteriol 2007 May;189(9):3639-44
PubMed ID: 17337576
Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS.
Wong YC, Abd El Ghany M, Naeem R, Lee KW, Tan YC, Pain A, Nathan S
Front Microbiol 2016;7:1288
PubMed ID: 27597847