Burkholderia glumae BGR1, bglu_2g06400 (toxA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia glumae BGR1
GCF_000022645.2|latest
Locus Tag
bglu_2g06400
NCBI Locus TagBGLU_RS21055
Name
toxA
Synonym: TRP-1
Replicon chromosome 2
Genomic location 769152 - 769889 (+ strand)
CommentFurther details in Identification of Proteins Involved in Toxin Production by Pseudomonas glumae. published in Japanese Journal of Phytopathology 64(2):75-79,January 1998.
CommentMember of the toxoflavin biosynthesis gene cluster

Cross-References

RefSeq WP_012733471.1
GI
Entrez 7904514
NCBI Locus Tag BGLU_RS21055
NCBI Old Locus Tag bglu_2g06400
RefSeq WP_012733471.1,GeneID

Product

Feature Type CDS
Coding Frame 1
Product Name
methyltransferase ToxA
Product Name Confidence: Class 1
Synonyms putative ubiquinone/menaquinone biosynthesis methyltransferase
Evidence for Translation
Charge (pH 7) -5.89
Kyte-Doolittle Hydrophobicity Value -0.269
Molecular Weight (kDa) 27591.9
Isoelectric Point (pI) 5.16

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5JE0 TRANSFERASE 04/17/16 Crystal structure of Burkholderia glumae ToxA with bound S-adenosylhomocysteine (SAH) and 1,6-didemethyltoxoflavin BURKHOLDERIA GLUMAE 1.552 X-RAY DIFFRACTION 100.0
5JE2 TRANSFERASE 04/17/16 Crystal structure of Burkholderia glumae ToxA Y7F mutant with bound S-adenosylhomocysteine (SAH) BURKHOLDERIA GLUMAE 1.519 X-RAY DIFFRACTION 99.6
5JE3 TRANSFERASE 04/17/16 Crystal structure of Burkholderia glumae ToxA Y7A mutant with bound S-adenosylhomocysteine (SAH) BURKHOLDERIA GLUMAE 1.792 X-RAY DIFFRACTION 99.6
5JE1 TRANSFERASE 04/17/16 Crystal structure of Burkholderia glumae ToxA with bound S-adenosylhomocysteine (SAH) and toxoflavin BURKHOLDERIA GLUMAE 1.95 X-RAY DIFFRACTION 100.0
5JE4 TRANSFERASE 04/17/16 Crystal structure of Burkholderia glumae ToxA Y7A mutant with bound S-adenosylhomocysteine (SAH) BURKHOLDERIA GLUMAE 1.932 X-RAY DIFFRACTION 99.6
5JDZ TRANSFERASE 04/17/16 Crystal structure of Burkholderia glumae ToxA with bound S-adenosylhomocysteine (SAH) BURKHOLDERIA GLUMAE 1.6 X-RAY DIFFRACTION 100.0
5JDY TRANSFERASE 04/17/16 Crystal structure of Burkholderia glumae ToxA Y7F mutant with bound S-adenosylhomocysteine (SAH) and toxoflavin BURKHOLDERIA GLUMAE 1.77 X-RAY DIFFRACTION 99.6
5JE5 TRANSFERASE 04/17/16 Crystal structure of Burkholderia glumae ToxA with bound S-adenosylhomocysteine (SAH) and 1-demethyltoxoflavin BURKHOLDERIA GLUMAE 1.564 X-RAY DIFFRACTION 100.0
5JE6 TRANSFERASE 04/17/16 Crystal structure of Burkholderia glumae ToxA BURKHOLDERIA GLUMAE 1.571 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 285 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG035431 (6 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: bglu_2g06400
Search term: toxA

Human Homologs

References

Burkholderia glumae ToxA Is a Dual-Specificity Methyltransferase That Catalyzes the Last Two Steps of Toxoflavin Biosynthesis.
Fenwick MK, Philmus B, Begley TP, Ealick SE
Biochemistry 2016 May 17;55(19):2748-59
PubMed ID: 27070241