Burkholderia cenocepacia J2315, BCAS0409 (zmpA)

Gene Feature Overview

Strain	Burkholderia cenocepacia J2315 GCF_000009485.1|latest
Locus Tag	BCAS0409
NCBI Locus Tag	QU43_RS70715
Name	zmpA
Replicon	chromosome 3
Genomic location	478548 - 480245 (+ strand)

Cross-References

RefSeq	WP_006481898.1
GI	197295256
Entrez	6804294
GI	197295256
INSDC	CAR57339.1
NCBI Locus Tag	QU43_RS70715
NCBI Old Locus Tag	BCAS0409
RefSeq	WP_006481898.1,GeneID
UniParc	UPI0000663A8F
UniProtKB Acc	B4ENF7
UniProtKB ID	B4ENF7_BURCJ
UniRef100	UniRef100_Q2VL23
UniRef50	UniRef50_Q2VL22
UniRef90	UniRef90_Q2VL22

Product

Feature Type	CDS
Coding Frame	1
Product Name	zinc metalloprotease ZmpA
Synonyms
Evidence for Translation
Charge (pH 7)	3.58
Kyte-Doolittle Hydrophobicity Value	-0.282
Molecular Weight (kDa)	59904.0
Isoelectric Point (pI)	8.18

Subcellular localization

Individual Mappings	Localization Confidence PMID Extracellular Class 3 20472543 Extracellular Class 1 Extracellular Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database	BGD
Category	ZmpA protease
Evidence	ECO:0000250 sequence similarity evidence used in manual assertion
Host Organism	Rattus norvegicus
Notes	zmpA mutant in K56-2 is attenuated for virulence in agar bead model of chronic lung infection
Reference	12904566

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 111 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group	BG003484 (514 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: BCAS0409 Search term: zmpA Search term: zinc metalloprotease ZmpA

Human Homologs

References

Burkholderia cenocepacia type VI secretion system mediates escape of type II secreted proteins into the cytoplasm of infected macrophages. Rosales-Reyes R, Aubert DF, Tolman JS, Amer AO, Valvano MA PLoS One 2012;7(7):e41726 PubMed ID: 22848580

An extracellular zinc metalloprotease gene of Burkholderia cepacia. Corbett CR, Burtnick MN, Kooi C, Woods DE, Sokol PA Microbiology 2003 Aug;149(Pt 8):2263-2271 PubMed ID: 12904566

Purification and characterization of an extracellular protease from Pseudomonas cepacia. McKevitt AI, Bajaksouzian S, Klinger JD, Woods DE Infect Immun 1989 Mar;57(3):771-8 PubMed ID: 2645209