Burkholderia cenocepacia J2315, BCAM2761 (cblA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAM2761
NCBI Locus TagQU43_RS68265
Name
cblA
Replicon chromosome 2
Genomic location 3125238 - 3125738 (- strand)

Cross-References

RefSeq WP_006485048.1
GI 206564599
Entrez 6930409
GI 206564599
INSDC CAR56625.1
NCBI Locus Tag QU43_RS68265
NCBI Old Locus Tag BCAM2761
RefSeq WP_006485048.1,GeneID
UniParc UPI00000B2588
UniProtKB Acc B4ELG0
UniProtKB ID B4ELG0_BURCJ
UniRef100 UniRef100_B4ELG0
UniRef50 UniRef50_B4ELG0
UniRef90 UniRef90_B4ELG0

Product

Feature Type CDS
Coding Frame 1
Product Name
giant cable pilus
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -1.02
Kyte-Doolittle Hydrophobicity Value 0.234
Molecular Weight (kDa) 17047.5
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Fimbrial
Note: This is based on PSORTb V3.0 secondary localization prediction
 Class 3
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database BGD
Category Cable pili
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Homo sapiens
Notes Cable pili are necessary for inducing A549 transformed human epithelial lung cell death via apoptosis
Reference 17537663

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 11 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG005919 (93 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAM2761
Search term: cblA
Search term: giant cable pilus

Human Homologs

References

The emergence of a highly transmissible lineage of cbl+ Pseudomonas (Burkholderia) cepacia causing CF centre epidemics in North America and Britain.
Sun L, Jiang RZ, Steinbach S, Holmes A, Campanelli C, Forstner J, Sajjan U, Tan Y, Riley M, Goldstein R
Nat Med 1995 Jul;1(7):661-6
PubMed ID: 7585148
Erratum in: J Bacteriol. 2009 Apr;191(8):2907.
The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients.
J Bacteriol. 2009 Jan;191(1):261-77.
PubMed ID: 18931103
Adherence and autoaggregation phenotypes of a Burkholderia cenocepacia cable pilus mutant.
Tomich M, Mohr CD
FEMS Microbiol Lett 2003 Nov 21;228(2):287-97
PubMed ID: 14638436
Cable pili and the 22-kilodalton adhesin are required for Burkholderia cenocepacia binding to and transmigration across the squamous epithelium.
Urban TA, Goldberg JB, Forstner JF, Sajjan US
Infect Immun 2005 Sep;73(9):5426-37
PubMed ID: 16113259
Identification and molecular analysis of cable pilus biosynthesis genes in Burkholderia cepacia.
Sajjan US, Xie H, Lefebre MD, Valvano MA, Forstner JF
Microbiology 2003 Apr;149(Pt 4):961-971
PubMed ID: 12686638
Pilus-mediated epithelial cell death in response to infection with Burkholderia cenocepacia.
Cheung KJ Jr, Li G, Urban TA, Goldberg JB, Griffith A, Lu F, Burns JL
Microbes Infect 2007 Jun;9(7):829-37
PubMed ID: 17537663