Burkholderia cenocepacia J2315, BCAL0404 (paaK1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAL0404
NCBI Locus TagQU43_RS38555
Name
paaK1
Synonym: paaK
Replicon chromosome 1
Genomic location 437425 - 438723 (- strand)

Cross-References

RefSeq WP_006491104.1
GI 206558810
Entrez 6930938
GI 206558810
INSDC CAR50715.1
NCBI Locus Tag QU43_RS38555
NCBI Old Locus Tag BCAL0404
RefSeq WP_006491104.1,GeneID
UniParc UPI00017B8EB7
UniProtKB Acc B4E7B5
UniProtKB ID B4E7B5_BURCJ
UniRef100 UniRef100_B4E7B5
UniRef50 UniRef50_Q9L9C1
UniRef90 UniRef90_B4E7B5

Product

Feature Type CDS
Coding Frame 1
Product Name
PaaK1
Product Name Confidence: Class 1
Synonyms phenylacetate-coenzyme A ligase
Evidence for Translation
Charge (pH 7) -6.12
Kyte-Doolittle Hydrophobicity Value -0.225
Molecular Weight (kDa) 47695.1
Isoelectric Point (pI) 5.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2Y27 LIGASE 12/13/10 crystal structure of PaaK1 in complex with ATP from Burkholderia cenocepacia BURKHOLDERIA CENOCEPACIA 1.6 X-RAY DIFFRACTION 100.0
2Y4N LIGASE 01/07/11 PaaK1 in complex with phenylacetyl adenylate BURKHOLDERIA CENOCEPACIA 1.92 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 318 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000429 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAL0404
Search term: paaK1
Search term: PaaK1

Human Homologs

References

Defining a structural and kinetic rationale for paralogous copies of phenylacetate-CoA ligases from the cystic fibrosis pathogen Burkholderia cenocepacia J2315.
Law A, Boulanger MJ
J Biol Chem 2011 Apr 29;286(17):15577-85
PubMed ID: 21388965