Burkholderia thailandensis E264; ATCC 700388, BTH_I2900 (pacL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia thailandensis E264; ATCC 700388
Locus Tag
BTH_I2900
NCBI Locus TagBTH_RS26975
Name
pacL
Synonym: putative 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase
Synonym: paaN
Replicon chromosome I
Genomic location 3334776 - 3336482 (+ strand)
Transposon Mutants 3 transposon mutants in E264; ATCC 700388

Cross-References

RefSeq YP_443407.1
GI 83718561
Entrez 3847097
GI 83718561
NCBI Locus Tag BTH_RS26975
NCBI Old Locus Tag BTH_I2900
RefSeq WP_009888721.1
UniProtKB Acc Q2SUJ2
UniProtKB ID Q2SUJ2_BURTA

Product

Feature Type CDS
Coding Frame 1
Product Name
PacL
Product Name Confidence: Class 2
Synonyms phenylacetic acid degradation protein paaN
CommentIn E. coli, paaZ encodes a bifunctional protein consisting of an N-terminal NADP-dependent aldehyde dehydrogenase domain and a C-terminal MaoC-like (R)-specific enoyl-CoA hydratase domain. In Burkholderia genomes, a gene incorrectly annotated as paaZ (e.g WQ49_RS11500) encodes only one of the two domains found in E.coli paaZ (the domain with semialdehyde dehydrogenase activity) and so is renamed PacL (PMID:21296885) but was also referred to as PaaZ-ALDH in the manuscript. In the same study , a separate A. aromaticum, protein (UniProtKB:Q5P232) with the (R)-hydratase activity was annotated as Ech-Aa and the Burkholderia ortholog was annotated as PaaZ-ECH.
Evidence for Translation
Charge (pH 7) -3.99
Kyte-Doolittle Hydrophobicity Value -0.040
Molecular Weight (kDa) 60364.7
Isoelectric Point (pI) 6.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 467 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000433 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BTH_I2900
Search term: pacL
Search term: PacL

References

PubMed ID: 21296885
A functional phenylacetic acid catabolic pathway is required for full pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans host model.
Law RJ(1), Hamlin JN, Sivro A, McCorrister SJ, Cardama GA, Cardona ST.
J Bacteriol. 2008 Nov;190(21):7209-18.
PubMed ID: 18776009