Burkholderia thailandensis E264 ATCC 700388, BTH_II0861 (tagB-5)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia thailandensis E264 ATCC 700388
GCF_000012365.1|latest
Locus Tag
BTH_II0861
NCBI Locus TagBTH_RS04470
Name
tagB-5
Replicon chromosome II
Genomic location 1005686 - 1006750 (- strand)
CommentThis gene is part of cluster known as the type VI secretion system gene cluster tss-5 based on naming convention used for B. pseudomallei described in PMID:17660433, however unlike B. pseudomallei, the numbers following the vgrG and hcp gene match the cluster number (PMID:20865170). While other T6SSs were dispensible, T6SS-5 is required for virulence in a murine acute melioidosis model (PMID:20865170).

Cross-References

RefSeq YP_439058.1
GI 83717467
Entrez 3846429
GI 83717467
NCBI Locus Tag BTH_RS04470
NCBI Old Locus Tag BTH_II0861
RefSeq WP_009896184.1
UniProtKB Acc Q2T6Z1
UniProtKB ID Q2T6Z1_BURTA

Product

Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system-associated protein TagB-5
Product Name Confidence: Class 2
Synonyms pentapeptide repeat-containing protein
TagB-5
Evidence for Translation
Charge (pH 7) -7.74
Kyte-Doolittle Hydrophobicity Value -0.035
Molecular Weight (kDa) 37585.3
Isoelectric Point (pI) 5.76

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0429
Category T6SS-1
Evidence ECO:0000250
sequence similarity evidence used in manual assertion
Host Organism
Notes
Annotation transferred from Burkholderia pseudomallei K96243
Reference 17984080

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 232 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG020940 (317 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BTH_II0861
Search term: tagB-5

Human Homologs

References

Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD
PLoS Pathog 2010 Aug 26;6(8):e1001068
PubMed ID: 20865170
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434