Burkholderia thailandensis E264 ATCC 700388, BTH_II0140 (clpV-2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia thailandensis E264 ATCC 700388
GCF_000012365.1|latest
Locus Tag
BTH_II0140
NCBI Locus TagBTH_RS00715
Name
clpV-2
Replicon chromosome II
Genomic location 166857 - 169613 (+ strand)
Transposon Mutants 3 transposon mutants in E264 ATCC 700388
CommentThis gene is part of cluster known as the type VI secretion system gene cluster tss-2 based on naming convention used for B. pseudomallei described in PMID:17660433, however unlike B. pseudomallei, the numbers following the vgrG and hcp gene match the cluster number (PMID:20865170).

Cross-References

RefSeq YP_438342.1
GI 83717355
Entrez 3846726
GI 83717355
INSDC ABC35244.1
NCBI Locus Tag BTH_RS00715
NCBI Old Locus Tag BTH_II0140
RefSeq YP_438342.1
UniParc UPI0000667762
UniProtKB Acc Q2T907
UniProtKB ID Q2T907_BURTA
UniRef100 UniRef100_Q2T907
UniRef50 UniRef50_Q9F746
UniRef90 UniRef90_Q2T907

Product

Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssH-2
Product Name Confidence: Class 2
Synonyms ClpA/B type protease
ClpV-2
Evidence for Translation
Charge (pH 7) -20.03
Kyte-Doolittle Hydrophobicity Value -0.038
Molecular Weight (kDa) 99533.4
Isoelectric Point (pI) 5.41

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 598 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG016113 (464 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BTH_II0140
Search term: clpV-2

Human Homologs

Ensembl 97, assembly GRCh38.p12
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 1.9e-29
Percent Identity: 30.5
Ensembl 97, assembly GRCh38.p12
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 1.9e-29
Percent Identity: 30.5
Ensembl 97, assembly GRCh38.p12
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 1.9e-29
Percent Identity: 30.5
Ensembl 97, assembly GRCh38.p12
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 1.9e-29
Percent Identity: 30.5
Ensembl 97, assembly GRCh38.p12
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 1.9e-29
Percent Identity: 30.5

References

Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD
PLoS Pathog 2010 Aug 26;6(8):e1001068
PubMed ID: 20865170
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434