Burkholderia thailandensis E264 ATCC 700388, BTH_II0126 (tssL-2b)

Gene Feature Overview

Strain	Burkholderia thailandensis E264 ATCC 700388 GCF_000012365.1|latest
Locus Tag	BTH_II0126
NCBI Locus Tag	BTH_RS00645
Name	tssL-2b
Replicon	chromosome II
Genomic location	145209 - 146510 (+ strand)
Transposon Mutants	3 transposon mutants in E264 ATCC 700388
Comment	This gene is part of cluster known as the type VI secretion system gene cluster tss-2 based on naming convention used for B. pseudomallei described in PMID:17660433, however unlike B. pseudomallei, the numbers following the vgrG and hcp gene match the cluster number (PMID:20865170).

Cross-References

RefSeq	YP_438328.1
GI	83716132
Entrez	3845788
GI	83716132
INSDC	AIP28311.1
NCBI Locus Tag	BTH_RS00645
NCBI Old Locus Tag	BTH_II0126
RefSeq	WP_009894733.1
UniParc	UPI00006672BC
UniProtKB Acc	Q2T921
UniProtKB ID	Q2T921_BURTA
UniRef100	UniRef100_Q2T921
UniRef50	UniRef50_B3R2S2
UniRef90	UniRef90_Q2T921

Product

Feature Type	CDS
Coding Frame	1
Product Name	type VI secretion system protein TssL-2 Product Name Confidence: Class 2
Synonyms	TssL-2b hypothetical protein
Evidence for Translation
Charge (pH 7)	-1.62
Kyte-Doolittle Hydrophobicity Value	-0.142
Molecular Weight (kDa)	47711.8
Isoelectric Point (pI)	6.54

Subcellular localization

Individual Mappings	Localization Confidence PMID Unknown (This protein may have multiple localization sites) Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 402 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group	BG002925 (1191 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: BTH_II0126 Search term: tssL-2b Search term: type VI secretion system protein TssL-2

Human Homologs

References

Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD PLoS Pathog 2010 Aug 26;6(8):e1001068 PubMed ID: 20865170

In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages. Shalom G, Shaw JG, Thomas MS Microbiology 2007 Aug;153(Pt 8):2689-2699 PubMed ID: 17660433

Strategies for Intracellular Survival of Burkholderia pseudomallei. Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD Front Microbiol 2011;2:170 PubMed ID: 22007185

Type VI secretion is a major virulence determinant in Burkholderia mallei. Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D Mol Microbiol 2007 Jun;64(6):1466-85 PubMed ID: 17555434