Burkholderia pseudomallei K96243, BPSS1722 (mdh)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
Locus Tag
BPSS1722
Name
mdh
Replicon chromosome 2
Genomic location 2360644 - 2361627 (+ strand)

Cross-References

RefSeq YP_111728.1
GI 53722743
Entrez 3095126
GI 53722743
INSDC CAH39196.1
NCBI Locus Tag BPSS1722
RefSeq YP_111728.1,GeneID
UniParc UPI000043BDD9
UniProtKB Acc P80536
UniProtKB ID MDH_BURPS
UniRef100 UniRef100_A3MBR2
UniRef50 UniRef50_A1W9K7
UniRef90 UniRef90_Q2T7J2

Product

Feature Type CDS
Coding Frame 1
Product Name
malate dehydrogenase
Synonyms
Evidence for Translation
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (32.8 kDa, pI 6.01, PMID:21960737)
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (32.2 kDa, pI 4.46, PMID:21960737)
Charge (pH 7) -3.09
Kyte-Doolittle Hydrophobicity Value 0.006
Molecular Weight (kDa) 35014.9
Isoelectric Point (pI) 5.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
quantitative mass spectrometry evidence
Unknown Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3D5T OXIDOREDUCTASE 05/16/08 Crystal structure of malate dehydrogenase from Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI 2.51 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 414 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG003064 (654 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1722
Search term: mdh

Human Homologs

Ensembl 86, assembly GRCh38.p7
malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGNC:6970]
E-value: 4.7e-86
Percent Identity: 52.4

References

Quantitative Proteomic Analysis of Burkholderia pseudomallei Bsa Type III Secretion System Effectors Using Hypersecreting Mutants.
Vander Broek CW(1), Chalmers KJ(2), Stevens MP(1), Stevens JM(3).
Mol Cell Proteomics. 2015 Apr;14(4):905-16.
PubMed ID: 25635268