Burkholderia pseudomallei K96243, BPSS1534 (bsaZ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS1534
Name
bsaZ
Synonym: sctU
Replicon chromosome 2
Genomic location 2088082 - 2089317 (- strand)
CommentThis gene is part of the type III secretion system T3SS-3 (bsa) gene locus.

Cross-References

RefSeq YP_111540.1
GI 53722555
Entrez 3096627
GI 53722555
INSDC CAH39007.1
NCBI Locus Tag BPSS1534
RefSeq YP_111540.1,GeneID
UniParc UPI000043BD20
UniProtKB Acc Q63K32
UniProtKB ID BSAZ_BURPS
UniRef100 UniRef100_Q63K32
UniRef50 UniRef50_Q63K32
UniRef90 UniRef90_Q63K32

Product

Feature Type CDS
Coding Frame 1
Product Name
type III secretion system export apparatus protein BsaZ (T3SS-3)
Product Name Confidence: Class 1
Synonyms surface presentation of antigens protein SpaS
Evidence for Translation
Charge (pH 7) 5.05
Kyte-Doolittle Hydrophobicity Value 0.070
Molecular Weight (kDa) 44457.8
Isoelectric Point (pI) 8.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0428
Category T3SS-3
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Notes
Reference 17984080
Source 2
Database Victors : 1987
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes bsaZ mutant is attenuated in mouse macrophages
Reference 15289563
Source 3
Database BGD
Category T3SS-3
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes bsaZ mutant has reduced intracellular replication in J774.2 murine macrophage-like cells
Reference 12410823

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 320 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019837 (326 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1534
Search term: bsaZ

Human Homologs

References

Unraveling type III secretion systems in the highly versatile Burkholderia pseudomallei.
Sun GW, Gan YH
Trends Microbiol 2010 Dec;18(12):561-8
PubMed ID: 20951592
Type III Secretion in the Melioidosis Pathogen <i>Burkholderia pseudomallei</i>.
Vander Broek CW, Stevens JM
Front Cell Infect Microbiol 2017;7:255
PubMed ID: 28664152
Burkholderia pseudomallei type III secretion system mutants exhibit delayed vacuolar escape phenotypes in RAW 264.7 murine macrophages.
Burtnick MN, Brett PJ, Nair V, Warawa JM, Woods DE, Gherardini FC
Infect Immun 2008 Jul;76(7):2991-3000
PubMed ID: 18443088
Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268
An Inv/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen.
Stevens MP, Wood MW, Taylor LA, Monaghan P, Hawes P, Jones PW, Wallis TS, Galyov EE
Mol Microbiol 2002 Nov;46(3):649-59
PubMed ID: 12410823