Burkholderia pseudomallei K96243, BPSS1529 (bipD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS1529
Name
bipD
Synonym: ipaD
Synonym: sctA
Replicon chromosome 2
Genomic location 2082841 - 2083773 (- strand)
CommentThis gene is part of the type III secretion system T3SS-3 (bsa) gene locus.

Cross-References

RefSeq YP_111535.1
GI 53722550
Entrez 3096115
GI 53722550
INSDC CAH39002.1
NCBI Locus Tag BPSS1529
RefSeq YP_111535.1,GeneID
UniParc UPI000043BD1B
UniProtKB Acc Q63K37
UniProtKB ID BIPD_BURPS
UniRef100 UniRef100_A3MCH1
UniRef50 UniRef50_Q2T708
UniRef90 UniRef90_A3MCH1

Product

Feature Type CDS
Coding Frame 1
Product Name
type III secretion system needle tip protein BipD (T3SS-3)
Product Name Confidence: Class 1
Synonyms membrane antigen
Evidence for Translation
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (30.0 kDa, pI 4.95, PMID:21960737)
Charge (pH 7) -7.03
Kyte-Doolittle Hydrophobicity Value -0.362
Molecular Weight (kDa) 33975.1
Isoelectric Point (pI) 4.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3NFT TRANSPORT PROTEIN 06/10/10 Near-atomic resolution analysis of BipD- A component of the type-III secretion system of Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI 1.51 X-RAY DIFFRACTION 99.7
2J9T TOXIN 11/16/06 BipD of Burkholderia Pseudomallei BURKHOLDERIA PSEUDOMALLEI 2.7 X-RAY DIFFRACTION 99.7
2IZP TOXIN 07/25/06 BipD - an invasion protein associated with the type-III secretion system of Burkholderia pseudomallei. BURKHOLDERIA PSEUDOMALLEI 2.1 X-RAY DIFFRACTION 99.7
2IXR TOXIN 07/10/06 BipD of Burkholderia Pseudomallei BURKHOLDERIA PSEUDOMALLEI 2.6 X-RAY DIFFRACTION 99.7

Virulence Evidence

Source 1
Database VFDB : VF0428
Category T3SS-3
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Notes
Reference 17984080
Source 2
Database Victors : 1984
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes bipD mutant was attenuated in mice
Reference 16987331
Source 3
Database BGD
Category T3SS-3
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes bipD mutant has reduced intracellular replication in J774.2 murine macrophage-like cells
Reference 12410823

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 10 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019830 (320 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1529
Search term: bipD

Human Homologs

References

Unraveling type III secretion systems in the highly versatile Burkholderia pseudomallei.
Sun GW, Gan YH
Trends Microbiol 2010 Dec;18(12):561-8
PubMed ID: 20951592
Near-atomic resolution analysis of BipD, a component of the type III secretion system of Burkholderia pseudomallei.
Pal M, Erskine PT, Gill RS, Wood SP, Cooper JB
Acta Crystallogr Sect F Struct Biol Cryst Commun 2010 Sep 1;66(Pt 9):990-3
PubMed ID: 20823511
An Inv/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen.
Stevens MP, Wood MW, Taylor LA, Monaghan P, Hawes P, Jones PW, Wallis TS, Galyov EE
Mol Microbiol 2002 Nov;46(3):649-59
PubMed ID: 12410823
Type III Secretion in the Melioidosis Pathogen <i>Burkholderia pseudomallei</i>.
Vander Broek CW, Stevens JM
Front Cell Infect Microbiol 2017;7:255
PubMed ID: 28664152
Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268