Burkholderia pseudomallei K96243, BPSS1509 (tssK-5)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
Locus Tag
BPSS1509
Name
tssK-5
Replicon chromosome 2
Genomic location 2058639 - 2060036 (+ strand)
CommentGene is part of a cluster known as the type VI secretion system gene cluster tss-5 used in much of the early P. pseudomallei literature (PMID:17660433) but later referred to as T6SS-1 cluster later (PMID:21670170, PMID:22007185) and by the B. mallei literature (PMID:22892068, PMID:17555434).

Cross-References

RefSeq YP_111516.1
GI 53722531
Entrez 3095047
GI 53722531
INSDC CAH38982.1
NCBI Locus Tag BPSS1509
RefSeq YP_111516.1,GeneID
UniParc UPI000043BD08
UniProtKB Acc Q63K56
UniProtKB ID Q63K56_BURPS
UniRef100 UniRef100_A3P6W1
UniRef50 UniRef50_A1UW72
UniRef90 UniRef90_A1UW72

Product

Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssK-5
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -3.59
Kyte-Doolittle Hydrophobicity Value -0.276
Molecular Weight (kDa) 51862.3
Isoelectric Point (pI) 6.64

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database Victors : 1981
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes BPSS1509 mutant is attenuated in mice
Reference 17189432
Source 2
Database VFDB : VF0429
Category T6SS-1
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Notes
Reference 17984080

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 101 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019349 (336 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1509
Search term: tssK-5

References

In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS.
Microbiology. 2007 Aug;153(Pt 8):2689-99.
PubMed ID: 17660433
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA(1), Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D.
Mol Microbiol. 2007 Jun;64(6):1466-85.
PubMed ID: 17555434
Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD.
PLoS Pathog. 2010 Aug 26;6(8)
PubMed ID: 20865170