Burkholderia pseudomallei K96243, BPSL3319 (fliC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL3319
Name
fliC
Replicon chromosome 1
Genomic location 3937675 - 3938841 (+ strand)

Cross-References

RefSeq YP_109915.1
GI 53720929
Entrez 3094015
GI 53720929
INSDC CAH37332.1
NCBI Locus Tag BPSL3319
RefSeq YP_109915.1,GeneID
UniParc UPI00000BEF3A
UniProtKB Acc H7C7G3
UniProtKB ID H7C7G3_BURPS
UniRef100 UniRef100_Q3JY46
UniRef50 UniRef50_Q3JY46
UniRef90 UniRef90_Q3JY46

Product

Feature Type CDS
Coding Frame 1
Product Name
flagellin
Synonyms
Evidence for Translation
Detected in B. pseudomallei strain DD503 using trypsin shaving and mass spectrometry (PMID:21391724). Found in at least two preparations.
Charge (pH 7) -2.01
Kyte-Doolittle Hydrophobicity Value -0.066
Molecular Weight (kDa) 39256.0
Isoelectric Point (pI) 4.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Flagellar
Note: This is based on PSORTb V3.0 secondary localization prediction
Class 3
Extracellular Class 1
quantitative mass spectrometry evidence
Additional evidence for subcellular localization
Detected in Triton-insoluble fraction of lysozyme-treated B. pseudomallei DD503 cells using trypsin shaving and mass spectrometry (PMID:21391724).

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4CFI STRUCTURAL PROTEIN 11/18/13 3D structure of FliC from Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI 1.3 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database Victors : 2010
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes fliC mutant is attenuated in mice
Reference 12183585
Source 2
Database VFDB : VF0430
Category Flagella
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Notes
Reference 17984080

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 302 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000148 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL3319
Search term: fliC
Search term: flagellin

Human Homologs

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268