Burkholderia pseudomallei K96243, BPSL3215 (tuf)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL3215
Name
tuf
Replicon chromosome 1
Genomic location 3809447 - 3810637 (- strand)

Cross-References

RefSeq YP_109809.1
GI 53720823
Entrez 3091614
GI 53720823
NCBI Locus Tag BPSL3215
RefSeq YP_109809.1,GeneID
UniProtKB Acc Q63PZ6
UniProtKB ID EFTU_BURPS

Product

Feature Type CDS
Coding Frame 1
Product Name
elongation factor Tu
Synonyms
Evidence for Translation
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (38.6 kDa, pI 4.94, PMID:21960737)
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (45.4 kDa, pI 4.97, PMID:21960737)
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (46.6 kDa, pI 4.93, PMID:21960737)
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (44.3 kDa, pI 4.68, PMID:21960737)
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (44.2 kDa, pI 4.10, PMID:21960737)
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (45.3 kDa, pI 4.16, PMID:21960737)
Charge (pH 7) -11.45
Kyte-Doolittle Hydrophobicity Value -0.148
Molecular Weight (kDa) 42990.9
Isoelectric Point (pI) 5.22

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Extracellular Class 1
quantitative mass spectrometry evidence
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000243 (1039 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL3215
Search term: tuf

Human Homologs

Ensembl 97, assembly GRCh38.p12
Tu translation elongation factor, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12420]
E-value: 5.5e-135
Percent Identity: 57.1

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268